Resources
Plasmid Files

pCLIP-Cas9-Nickase-TRE3G-Puro

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and a puromycin resistance marker expressed from a Tet-responsive promoter.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCLIP-Cas9(D10a)-Nickase-TRE3G-Puro.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
Download Free Trial Get SnapGene Viewer


SspI (10,676) PvuI (10,242) FspI (10,094) DrdI (9090) AflIII - PciI (8982) SmaI (8962) TspMI - XmaI (8960) AvrII (8939) PmeI (8614) PsiI (8564) KpnI (8229) Acc65I (8225) Bsu36I (8221) SalI (8101) EcoRI (7479) BstEII (7013) RsrII (6995) BsiWI (6935) PflFI - Tth111I (6921) BamHI (6816) BsaBI * (6138) PmlI (5429) SpeI (41) NdeI (276) SnaBI (382) NheI (405) BmtI (409) NruI * (867) BspQI - SapI (1136) FseI (1184) MfeI (1223) BbvCI (1458) AleI (1611) AbsI - PspXI (2238) XbaI (2623) AfeI (2630) PspOMI (3995) ApaI (3999) pCLIP-Cas9-Nickase-TRE3G-Puro 10,794 bp