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Plasmid Files

pCLIP-Cas9-Nickase-hCMV-tRFP

Lentiviral CRISPR vector encoding a Cas9 nickase mutant and the red fluorescent protein TurboRFP expressed from the human CMV promoter.

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pCLIP-Cas9(D10a)-Nickase-hCMV-tRFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
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SspI (11,040) PvuI (10,606) FspI (10,458) DrdI (9454) AflIII - PciI (9346) SmaI (9326) TspMI - XmaI (9324) AvrII (9303) SexAI * (9070) PmeI (8978) PsiI (8928) KpnI (8593) Acc65I (8589) SalI (8465) SacII (8370) PshAI (7774) SfiI (7610) BsrGI (7184) BsaBI * (6403) PmlI (5694) NheI (405) BmtI (409) KasI (671) NarI (672) SfoI (673) PluTI (675) BssHII (745) NruI * (867) BspQI - SapI (1136) MfeI (1219) BbvCI (1454) AleI (1607) BsmBI (2766) PspXI (2882) XbaI (2888) AfeI (2895) PspOMI (4260) ApaI (4264) pCLIP-Cas9-Nickase-hCMV-tRFP 11,158 bp