Resources
Plasmid Files

pCLIP-Cas9-Nuclease-TRE3G-tRFP

Lentiviral CRISPR vector encoding Cas9 and the red fluorescent protein TurboRFP expressed from a Tet-responsive promoter.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pCLIP-Cas9-Nuclease-TRE3G-tRFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  transOMIC
Download Free Trial Get SnapGene Viewer


SspI (10,775) PvuI (10,341) FspI (10,193) DrdI (9189) AflIII - PciI (9081) SmaI (9061) TspMI - XmaI (9059) AvrII (9038) SexAI * (8805) PmeI (8713) PsiI (8663) KpnI (8328) Acc65I (8324) SalI (8200) SacII (8105) EcoRI (7578) PshAI (7509) SfiI (7345) BsrGI (6919) BamHI (6816) BsaBI * (6138) SpeI (41) NdeI (276) SnaBI (382) NheI (405) BmtI (409) BssHII (745) NruI * (867) BspQI - SapI (1136) FseI (1184) MfeI (1223) BbvCI (1458) AleI (1611) AbsI - PspXI (2238) XbaI (2623) AfeI (2630) PspOMI (3995) ApaI (3999) PmlI (5429) pCLIP-Cas9-Nuclease-TRE3G-tRFP 10,893 bp
SspI  (10,775)
1 site
A A T A T T T T A T A A
PvuI  (10,341)
1 site
C G A T C G G C T A G C
FspI  (10,193)
1 site
T G C G C A A C G C G T
DrdI  (9189)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AflIII  (9081)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (9081)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SmaI  (9061)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (9059)
1 site
C C C G G G G G G C C C
XmaI  (9059)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
AvrII  (9038)
1 site
C C T A G G G G A T C C
SexAI  (8805)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
PmeI  (8713)
1 site
G T T T A A A C C A A A T T T G
PsiI  (8663)
1 site
T T A T A A A A T A T T
KpnI  (8328)
1 site
G G T A C C C C A T G G
Acc65I  (8324)
1 site
G G T A C C C C A T G G
SalI  (8200)
1 site
G T C G A C C A G C T G
SacII  (8105)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
EcoRI  (7578)
1 site
G A A T T C C T T A A G
PshAI  (7509)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SfiI  (7345)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G