pCLIP-gRNA-EFS-Blast

Lentiviral CRISPR vector encoding a guide RNA (gRNA), plus a blasticidin resistance marker expressed from the EF-1α core promoter.

Sequence Author: transOMIC

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polIII terminator BsmBI (2505) BsmBI (4390) pCLIP-gRNA-EFS-Blast 8465 bp
BsmBI  (2505)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (4390)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Stuffer
2506 .. 4390  =  1885 bp
Stuffer
2506 .. 4390  =  1885 bp
AmpR
7470 .. 8330  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7470 .. 8261  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7470 .. 8330  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8262 .. 8330  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7470 .. 8330  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
WPRE
5171 .. 5759  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
5171 .. 5759  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
6714 .. 7299  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6714 .. 7299  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BSD
4759 .. 5157  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
4759 .. 5157  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
SV40
6296 .. 6625  =  330 bp
SV40 enhancer and early promoter
SV40
6296 .. 6625  =  330 bp
SV40 enhancer and early promoter
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
U6 promoter
2261 .. 2501  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
2261 .. 2501  =  241 bp
RNA polymerase III promoter for human U6 snRNA
RRE
1333 .. 1566  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1333 .. 1566  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
3' LTR (ΔU3)
5967 .. 6200  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
5967 .. 6200  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
EF-1α core promoter
4528 .. 4739  =  212 bp
core promoter for human elongation factor EF-1α
EF-1α core promoter
4528 .. 4739  =  212 bp
core promoter for human elongation factor EF-1α
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2093 .. 2210  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2093 .. 2210  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
8331 .. 8435  =  105 bp
AmpR promoter
8331 .. 8435  =  105 bp
gRNA scaffold
4391 .. 4466  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
4391 .. 4466  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
4467 .. 4472  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
4467 .. 4472  =  6 bp
RNA polymerase III transcription terminator
SV40 ori
6476 .. 6611  =  136 bp
SV40 origin of replication
SV40 ori
6476 .. 6611  =  136 bp
SV40 origin of replication
ORF:  4759 .. 5157  =  399 bp
ORF:  132 amino acids  =  13.7 kDa
ORF:  7600 .. 7866  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1211 .. 2098  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  5093 .. 5788  =  696 bp
ORF:  231 amino acids  =  25.4 kDa
ORF:  5274 .. 5570  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  4425 .. 4760  =  336 bp
ORF:  111 amino acids  =  11.8 kDa
ORF:  7470 .. 8330  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  8227 .. 31  =  270 bp
ORF:  89 amino acids  =  10.4 kDa
ORF:  2684 .. 3079  =  396 bp
ORF:  131 amino acids  =  14.0 kDa
ORF:  1126 .. 1506  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
ORF:  4480 .. 5061  =  582 bp
ORF:  193 amino acids  =  21.5 kDa
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Download pCLIP-gRNA-EFS-Blast.dna file

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