pCS2-nCas9n

Plasmid for expressing zebrafish codon-optimized S. pyogenes Cas9 with N- and C-terminal nuclear localization signals.

Sequence Author: Chen Lab / Addgene #47929

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NotI (4523) ATG pCS2-nCas9n 8272 bp
NotI  (4523)
1 site
G C G G C C G C C G C C G G C G
ATG
105 .. 107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
105 .. 107  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
117 .. 137  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
117 .. 137  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
144 .. 4244  =  4101 bp
1367 amino acids  =  158.2 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
144 .. 4244  =  4101 bp
1367 amino acids  =  158.2 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4260 .. 4280  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4260 .. 4280  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
CMV IE94 promoter
7288 .. 8272  =  985 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
CMV IE94 promoter
7288 .. 8272  =  985 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
AmpR
5768 .. 6628  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5768 .. 6559  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5768 .. 6628  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6560 .. 6628  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5768 .. 6628  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5009 .. 5597  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5009 .. 5597  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
6759 .. 7214  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6759 .. 7214  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 poly(A) signal
4321 .. 4455  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4321 .. 4455  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6629 .. 6733  =  105 bp
AmpR promoter
6629 .. 6733  =  105 bp
SP6 promoter
35 .. 53  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
35 .. 53  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  7306 .. 7554  =  249 bp
ORF:  82 amino acids  =  9.1 kDa
ORF:  7954 .. 8226  =  273 bp
ORF:  90 amino acids  =  9.5 kDa
ORF:  105 .. 4283  =  4179 bp
ORF:  1392 amino acids  =  161.0 kDa
ORF:  5898 .. 6164  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7323 .. 7721  =  399 bp
ORF:  132 amino acids  =  14.3 kDa
ORF:  5768 .. 6628  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  6997 .. 7293  =  297 bp
ORF:  98 amino acids  =  10.6 kDa
ORF:  1764 .. 2066  =  303 bp
ORF:  100 amino acids  =  11.3 kDa
ORF:  2166 .. 2660  =  495 bp
ORF:  164 amino acids  =  18.5 kDa
ORF:  2865 .. 3095  =  231 bp
ORF:  76 amino acids  =  8.6 kDa
ORF:  3456 .. 4187  =  732 bp
ORF:  243 amino acids  =  27.8 kDa
ORF:  7227 .. 7583  =  357 bp
ORF:  118 amino acids  =  13.0 kDa
ORF:  7926 .. 8207  =  282 bp
ORF:  93 amino acids  =  10.1 kDa
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