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pFGC-pcoCas9

Binary plant vector for expressing plant codon-optimized Case9 from the 35SPPDK promoter.

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pFGC-pcoCas9.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Sheen Lab / Addgene #52256
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AanI (11,623) EcoRI (9934) PaeR7I - PspXI - XhoI (9928) SnaBI (9924) SwaI (9911) XbaI (9902) AscI (9895) I-PpoI (9882) PacI (9863) I-SceI (9853) I-CeuI (9826) Bsu36I (9822) PI-PspI (9801) SbfI (9751) NruI (9742) AsiSI (9736) SmaI - SrfI (9721) TspMI - XmaI (9719) AanI (6692) NcoI (5003) AanI (6670) pFGC-pcoCas9 13,330 bp
AanI  (11,623)
3 sites
T T A T A A A A T A T T
EcoRI  (9934)
1 site
G A A T T C C T T A A G
PaeR7I  (9928)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (9928)
1 site
V C T C G A G B B G A G C T C V
XhoI  (9928)
1 site
C T C G A G G A G C T C
SnaBI  (9924)
1 site
T A C G T A A T G C A T
SwaI  (9911)
1 site
A T T T A A A T T A A A T T T A