pHAGE-TO-nmdCas9-3XGFP

Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of GFP.

Sequence Author: Pederson Lab / Addgene #64109

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
CMV promoter tet operator tet operator ATG pHAGE-TO-nmdCas9-3XGFP 12,122 bp
ATG
2980 .. 2982  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2980 .. 2982  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
SV40 NLS
2986 .. 3006  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
2986 .. 3006  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 1:  
   3019 .. 3060  =  42 bp
   14 amino acids  =  1.5 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 2:  
   3061 .. 3063  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 3:  
   3064 .. 4773  =  1710 bp
   570 amino acids  =  65.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 4:  
   4774 .. 4779  =  6 bp
   2 amino acids  =  160.2 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 5:  
   4780 .. 4845  =  66 bp
   22 amino acids  =  2.5 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 6:  
   4846 .. 4848  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
   Segment 7:  
   4849 .. 6261  =  1413 bp
   471 amino acids  =  54.7 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
NmdCas9
3019 .. 6261  =  3243 bp
1081 amino acids  =  124.0 kDa
7 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Neisseria meningitidis Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the mutations D16A, D587A, H588A, and N611A
SV40 NLS
6280 .. 6300  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
6280 .. 6300  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
superfolder GFP
6313 .. 7026  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
superfolder GFP
6313 .. 7026  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
superfolder GFP
7045 .. 7758  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
superfolder GFP
7045 .. 7758  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
superfolder GFP
7777 .. 8490  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
superfolder GFP
7777 .. 8490  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
AmpR
9718 .. 10,578  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   9718 .. 9786  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9718 .. 10,578  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   9787 .. 10,578  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
9718 .. 10,578  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
1 .. 634  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
1 .. 634  =  634 bp
long terminal repeat (LTR) from HIV-1
WPRE
8512 .. 9100  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
8512 .. 9100  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
10,749 .. 11,337  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
10,749 .. 11,337  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
2299 .. 2602  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2299 .. 2602  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
LTR (ΔU3)
9175 .. 9408  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
9175 .. 9408  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
CMV promoter
2603 .. 2806  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2603 .. 2806  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2058 .. 2175  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2058 .. 2175  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
9613 .. 9717  =  105 bp
AmpR promoter
9613 .. 9717  =  105 bp
tet operator
2808 .. 2826  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2808 .. 2826  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2829 .. 2847  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2829 .. 2847  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  2980 .. 8499  =  5520 bp
ORF:  1839 amino acids  =  208.7 kDa
ORF:  8602 .. 9114  =  513 bp
ORF:  170 amino acids  =  18.6 kDa
ORF:  9718 .. 10,578  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  1181 .. 2107  =  927 bp
ORF:  308 amino acids  =  35.4 kDa
ORF:  2762 .. 3130  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  8615 .. 9148  =  534 bp
ORF:  177 amino acids  =  18.8 kDa
ORF:  1881 .. 2129  =  249 bp
ORF:  82 amino acids  =  9.0 kDa
ORF:  4071 .. 4307  =  237 bp
ORF:  78 amino acids  =  9.1 kDa
ORF:  10,182 .. 10,448  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  2875 .. 8469  =  5595 bp
ORF:  1864 amino acids  =  198.3 kDa
Click here to try SnapGene

Download pHAGE-TO-nmdCas9-3XGFP.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.