pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Vector encoding a single-chain anti-GCN4 peptide antibody fused to superfolder GFP, the VP64 activation domain, and the GB1 solubilization domain.

Sequence Author: Vale Lab / Addgene #60904

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS 10,074 bp
scFv GCN4
7811 .. 8551  =  741 bp
247 amino acids  =  25.8 kDa
Product: single-chain Fv antibody fragment against the GCN4 peptide
scFv GCN4
7811 .. 8551  =  741 bp
247 amino acids  =  25.8 kDa
Product: single-chain Fv antibody fragment against the GCN4 peptide
HA
8552 .. 8578  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
8552 .. 8578  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
superfolder GFP
8669 .. 9379  =  711 bp
237 amino acids  =  26.7 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
superfolder GFP
8669 .. 9379  =  711 bp
237 amino acids  =  26.7 kDa
Product: GFP variant that folds robustly even when fused to poorly folded proteins (Pédelacq et al., 2006)
VP64
9413 .. 9562  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
VP64
9413 .. 9562  =  150 bp
50 amino acids  =  5.5 kDa
Product: tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)
GB1
9593 .. 9754  =  162 bp
54 amino acids  =  6.0 kDa
Product: B1 domain of Streptococcal protein G  
effective as a solubilizing fusion partner (Cheng and Patel, 2004)
GB1
9593 .. 9754  =  162 bp
54 amino acids  =  6.0 kDa
Product: B1 domain of Streptococcal protein G  
effective as a solubilizing fusion partner (Cheng and Patel, 2004)
Rex NLS
9782 .. 9832  =  51 bp
17 amino acids  =  2.2 kDa
Product: nuclear localization signal from the human T-cell leukemia virus type 1 (HTLV-1) Rex protein
Rex NLS
9782 .. 9832  =  51 bp
17 amino acids  =  2.2 kDa
Product: nuclear localization signal from the human T-cell leukemia virus type 1 (HTLV-1) Rex protein
AmpR
1031 .. 1891  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1031 .. 1099  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1031 .. 1891  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1100 .. 1891  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1031 .. 1891  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
LTR
5302 .. 5935  =  634 bp
long terminal repeat (LTR) from HIV-1
LTR
5302 .. 5935  =  634 bp
long terminal repeat (LTR) from HIV-1
ori
2062 .. 2650  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2062 .. 2650  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2896 .. 3225  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2896 .. 3225  =  330 bp
SV40 enhancer and early promoter
RRE
6599 .. 6832  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
6599 .. 6832  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
LTR (ΔU3)
9935 .. 94  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
LTR (ΔU3)
9935 .. 94  =  234 bp
self-inactivating long terminal repeat (LTR) from HIV-1
SV40 promoter
7526 .. 7736  =  211 bp
SV40 early promoter
SV40 promoter
7526 .. 7736  =  211 bp
SV40 early promoter
SV40 poly(A) signal
4999 .. 5133  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4999 .. 5133  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
5982 .. 6107  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
5982 .. 6107  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
7346 .. 7463  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
7346 .. 7463  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
926 .. 1030  =  105 bp
AmpR promoter
926 .. 1030  =  105 bp
SV40 ori
3076 .. 3211  =  136 bp
SV40 origin of replication
SV40 ori
3076 .. 3211  =  136 bp
SV40 origin of replication
SV40 ori
7595 .. 7730  =  136 bp
SV40 origin of replication
SV40 ori
7595 .. 7730  =  136 bp
SV40 origin of replication
ORF:  3508 .. 3882  =  375 bp
ORF:  124 amino acids  =  14.0 kDa
ORF:  1031 .. 1891  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3662 .. 3943  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
ORF:  3974 .. 4357  =  384 bp
ORF:  127 amino acids  =  14.9 kDa
ORF:  7811 .. 9844  =  2034 bp
ORF:  677 amino acids  =  71.8 kDa
ORF:  327 .. 689  =  363 bp
ORF:  120 amino acids  =  12.4 kDa
ORF:  4503 .. 4976  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  6477 .. 7574  =  1098 bp
ORF:  365 amino acids  =  42.1 kDa
ORF:  1495 .. 1761  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3556 .. 3975  =  420 bp
ORF:  139 amino acids  =  15.9 kDa
ORF:  243 .. 554  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  6458 .. 6772  =  315 bp
ORF:  104 amino acids  =  11.0 kDa
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