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Plasmid Files

pICSL01009::AtU6p

Level 0 vector encoding a consensus Arabidopsis U6 promoter for expressing a single guide RNA (sgRNA).

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pICSL01009_AtU6p Sequence and MappICSL01009_AtU6p.dna
Map and Sequence File   
Sequence Author:  Kamoun Lab / Addgene #46968
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 BsaI (2224) BsaI (2148) pICSL01009::AtU6p 2323 bp
BsaI  (2224)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (2148)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
SmR
351 .. 1142  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase
(Murphy, 1985)
confers resistance to spectinomycin and
streptomycin
SmR
351 .. 1142  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase
(Murphy, 1985)
confers resistance to spectinomycin and
streptomycin
ori
1313 .. 1901  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1313 .. 1901  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AtU6 promoter
2153 .. 2227  =  75 bp
consensus promoter from the three Arabidopsis U6
snRNA genes (Waibel and Filipowicz, 1990;
Nekrasov et al., 2013)
AtU6 promoter
2153 .. 2227  =  75 bp
consensus promoter from the three Arabidopsis U6
snRNA genes (Waibel and Filipowicz, 1990;
Nekrasov et al., 2013)
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