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Plasmid Files

pLV hUbC-dCas9 VP64-T2A-GFP

Lentiviral vector for expressing enhanced GFP (EGFP) together with catalytically inactive dCas9 fused to the VP64 transcriptional activation domain.

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pLV hUbC-dCas9 VP64-T2A-GFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Gersbach Lab / Addgene #53192
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SgrDI (1) AhdI - AspEI - BmeRI - DriI - Eam1105I (14,017) BssNAI - Bst1107I - BstZ17I (12,745) FseI - RigI (12,385) SgrAI (12,223) MauBI (12,145) MssI - PmeI (10,932) AgeI - AsiGI - BshTI - CspAI - PinAI (9556) Bsp1407I - BsrGI - BstAUI - SspBI (9548) AscI - PalAI - SgsI (8429) FspAI (7424) MluI (231) BstSNI - Eco105I - SnaBI (593) CciNI - NotI (1525) BbvCI (1801) AbsI - PspXI (2881) XbaI (4357) SbfI - SdaI - Sse8387I (4721) XcmI (4825) BlpI - Bpu1102I - Bsp1720I - CelII (5422) BamHI (6479) BstXI (7263)