pLenti-Cas-Guide

Lentiviral vector for co-expressing a guide RNA (gRNA) together with Cas9, for genome editing using the S. pyogenes CRISPR/Cas9 system.

Sequence Author: OriGene

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
AmpR promoter FLAG SV40 NLS SV40 NLS cPPT/CTS CF3 Primer (2745 .. 2766) BamHI (2951) BsmBI (3001) polIII terminator CMV promoter T7 promoter pLenti-Cas-Guide 11,015 bp
BamHI  (2951)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsmBI  (3001)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
CF3 Primer
22-mer  /  45% GC
1 binding site
2745 .. 2766  =  22 annealed bases
Tm  =  56°C
Cas9
3814 .. 7917  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
3814 .. 7917  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
7918 .. 7938  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7918 .. 7938  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7945 .. 7965  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
7945 .. 7965  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Myc
7987 .. 8016  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
7987 .. 8016  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
FLAG
8035 .. 8058  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
8035 .. 8058  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
CmR
10,223 .. 10,882  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
10,223 .. 10,882  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
WPRE
8199 .. 8787  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
8199 .. 8787  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
9558 .. 10,146  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
9558 .. 10,146  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3128 .. 3507  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3128 .. 3507  =  380 bp
human cytomegalovirus immediate early enhancer
U6 promoter
2707 .. 2947  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
2707 .. 2947  =  241 bp
RNA polymerase III promoter for human U6 snRNA
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
CMV promoter
3508 .. 3711  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
3508 .. 3711  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early promoter
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
9316 .. 9496  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
9316 .. 9496  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
10,883 .. 10,987  =  105 bp
AmpR promoter
10,883 .. 10,987  =  105 bp
gRNA scaffold
3001 .. 3076  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
3001 .. 3076  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
T7 promoter
3737 .. 3755  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
3737 .. 3755  =  19 bp
promoter for bacteriophage T7 RNA polymerase
polIII terminator
3077 .. 3082  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
3077 .. 3082  =  6 bp
RNA polymerase III transcription terminator
ORF:  1561 .. 2448  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  3667 .. 8064  =  4398 bp
ORF:  1465 amino acids  =  169.8 kDa
ORF:  8302 .. 9342  =  1041 bp
ORF:  346 amino acids  =  38.3 kDa
ORF:  8289 .. 8843  =  555 bp
ORF:  184 amino acids  =  20.5 kDa
ORF:  1395 .. 1856  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  10,223 .. 10,882  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  4072 .. 4314  =  243 bp
ORF:  80 amino acids  =  9.6 kDa
ORF:  5866 .. 6210  =  345 bp
ORF:  114 amino acids  =  13.5 kDa
ORF:  6364 .. 6768  =  405 bp
ORF:  134 amino acids  =  15.6 kDa
ORF:  7237 .. 7860  =  624 bp
ORF:  207 amino acids  =  24.3 kDa
Click here to try SnapGene

Download pLenti-Cas-Guide.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.