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Plasmid Files

pMSCV-LTR-dCas9-VP64-BFP

Retroviral CRISPR activation vector for expressing TagBFP plus catalytically inactive dCas9 fused to the VP64 transcriptional activation domain.

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pMSCV-LTR-dCas9-VP64-BFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qi & Weissman Labs / Addgene #46912
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SgrAI (11,191) FauNDI - NdeI (10,819) BanIII - Bsa29I - BseCI - BshVI - BspDI - BspXI - Bsu15I - BsuTUI - ClaI (7686) Cfr42I - KspI - SacII - Sfr303I - SgrBI - SstII (7246) CpoI - CspI - RsrII - Rsr2I (7148) BsiWI - Pfl23II - PspLI (7088) Acc36I - BfuAI - BspMI - BveI (7021) AgeI - AsiGI - BshTI - CspAI - PinAI (6622) EcoRI (6502) PaeR7I - Sfr274I - SlaI - XhoI (6490) BamHI (6478) BoxI - BstPAI - PshAI (6406) BbsI - BpiI - BpuAI - BstV2I (6074) BglII (1410) Bsp19I - NcoI (1421) AccB7I - PflMI - Van91I (1722) SgrDI (1789) BtgZI (1973) BsaBI * - Bse8I - BseJI * - MamI * (2240) AanI - PsiI (2540) pMSCV-LTR-dCas9-VP64-BFP 11,369 bp <