Plasmid Files |
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![]() | pMZ374.dna |
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Efficient cleavage requires at least two copies of the CspCI recognition sequence. CspCI makes two double-stranded cuts.Sticky ends from different CspCI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the CspCI recognition sequence. CspCI makes two double-stranded cuts.Sticky ends from different CspCI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
Cas9 6911 .. 11,014 = 4104 bp 1368 amino acids = 158.4 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
Cas9 6911 .. 11,014 = 4104 bp 1368 amino acids = 158.4 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
AmpR 5155 .. 6015 = 861 bp 286 amino acids = 31.6 kDa Segment 2: 5155 .. 5946 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 5155 .. 6015 = 861 bp 286 amino acids = 31.6 kDa Segment 1: signal sequence 5947 .. 6015 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 5155 .. 6015 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
S. pombe ura4 1930 .. 2724 = 795 bp 264 amino acids = 29.4 kDa Product: orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis |
S. pombe ura4 1930 .. 2724 = 795 bp 264 amino acids = 29.4 kDa Product: orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis |
ars1 539 .. 1320 = 782 bp Schizosaccharomyces pombe autonomously replicating sequence ars1 |
ars1 539 .. 1320 = 782 bp Schizosaccharomyces pombe autonomously replicating sequence ars1 |
adh1 promoter 6149 .. 6890 = 742 bp promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase |
adh1 promoter 6149 .. 6890 = 742 bp promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase |
ori 4396 .. 4984 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4396 .. 4984 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
rrk1 promoter/leader 3601 .. 3957 = 357 bp promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014) |
rrk1 promoter/leader 3601 .. 3957 = 357 bp promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014) |
CYC1 terminator 11,063 .. 11,310 = 248 bp transcription terminator for CYC1 |
CYC1 terminator 11,063 .. 11,310 = 248 bp transcription terminator for CYC1 |
AmpR promoter 6016 .. 6120 = 105 bp |
AmpR promoter 6016 .. 6120 = 105 bp |
gRNA scaffold 3483 .. 3566 = 84 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
gRNA scaffold 3483 .. 3566 = 84 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
sTRSV HHRz 3431 .. 3482 = 52 bp hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003) |
sTRSV HHRz 3431 .. 3482 = 52 bp hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003) |
SV40 NLS 11,027 .. 11,047 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 11,027 .. 11,047 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
ORF: 1930 .. 2724 = 795 bp ORF: 264 amino acids = 29.4 kDa |
ORF: 3172 .. 3543 = 372 bp ORF: 123 amino acids = 14.1 kDa |
ORF: 6640 .. 6879 = 240 bp ORF: 79 amino acids = 8.9 kDa |
ORF: 5285 .. 5551 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 6911 .. 11,050 = 4140 bp ORF: 1379 amino acids = 159.7 kDa |
ORF: 6905 .. 7390 = 486 bp ORF: 161 amino acids = 18.0 kDa |
ORF: 8315 .. 8833 = 519 bp ORF: 172 amino acids = 20.1 kDa |
ORF: 8897 .. 9430 = 534 bp ORF: 177 amino acids = 20.0 kDa |
ORF: 10,424 .. 10,957 = 534 bp ORF: 177 amino acids = 20.0 kDa |
ORF: 2431 .. 2721 = 291 bp ORF: 96 amino acids = 10.7 kDa |
ORF: 5155 .. 6015 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 6778 .. 7053 = 276 bp ORF: 91 amino acids = 10.4 kDa |
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