Plasmid Files

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CspCI  (3567) 2 sites

Efficient cleavage requires at least two copies of the CspCI recognition sequence. CspCI makes two double-stranded cuts. Sticky ends from different CspCI sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

CspCI  (3601) 2 sites

Efficient cleavage requires at least two copies of the CspCI recognition sequence. CspCI makes two double-stranded cuts. Sticky ends from different CspCI sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

Cas9 6911 .. 11,014  =  4104 bp 1368 amino acids  =  158.4 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

Cas9 6911 .. 11,014  =  4104 bp 1368 amino acids  =  158.4 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

AmpR 5155 .. 6015  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      5155 .. 5946  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 5155 .. 6015  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      5947 .. 6015  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 5155 .. 6015  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

S. pombe ura4 1930 .. 2724  =  795 bp 264 amino acids  =  29.4 kDa Product: orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis

S. pombe ura4 1930 .. 2724  =  795 bp 264 amino acids  =  29.4 kDa Product: orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis

ars1 539 .. 1320  =  782 bp Schizosaccharomyces pombe autonomously replicating sequence ars1

ars1 539 .. 1320  =  782 bp Schizosaccharomyces pombe autonomously replicating sequence ars1

adh1 promoter 6149 .. 6890  =  742 bp promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase

adh1 promoter 6149 .. 6890  =  742 bp promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase

ori 4396 .. 4984  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 4396 .. 4984  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

rrk1 promoter/leader 3601 .. 3957  =  357 bp promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014)

rrk1 promoter/leader 3601 .. 3957  =  357 bp promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014)

CYC1 terminator 11,063 .. 11,310  =  248 bp transcription terminator for CYC1

CYC1 terminator 11,063 .. 11,310  =  248 bp transcription terminator for CYC1

AmpR promoter 6016 .. 6120  =  105 bp

AmpR promoter 6016 .. 6120  =  105 bp

gRNA scaffold 3483 .. 3566  =  84 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

gRNA scaffold 3483 .. 3566  =  84 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

sTRSV HHRz 3431 .. 3482  =  52 bp hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)

sTRSV HHRz 3431 .. 3482  =  52 bp hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)

SV40 NLS 11,027 .. 11,047  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 11,027 .. 11,047  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

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