Plasmid Files

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BsaI  (382) 2 sites

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

BsaI  (402) 2 sites

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

Cas9 1597 .. 5697  =  4101 bp 1367 amino acids  =  158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

Cas9 1597 .. 5697  =  4101 bp 1367 amino acids  =  158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

pVS1 RepA 8742 .. 9809  =  1068 bp 355 amino acids  =  39.7 kDa Product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

pVS1 RepA 8742 .. 9809  =  1068 bp 355 amino acids  =  39.7 kDa Product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

HygR 12,949 .. 13,974  =  1026 bp 341 amino acids  =  38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin

HygR 12,949 .. 13,974  =  1026 bp 341 amino acids  =  38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin

KanR 11,414 .. 12,208  =  795 bp 264 amino acids  =  31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin

KanR 11,414 .. 12,208  =  795 bp 264 amino acids  =  31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin

CaMV 35S promoter (enhanced) 14,042 .. 14,719  =  678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region

CaMV 35S promoter (enhanced) 14,042 .. 14,719  =  678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region

pVS1 StaA 7679 .. 8308  =  630 bp 209 amino acids  =  22.1 kDa Product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

pVS1 StaA 7679 .. 8308  =  630 bp 209 amino acids  =  22.1 kDa Product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

ori 10,739 .. 11,327  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 10,739 .. 11,327  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

OsU3 promoter 6 .. 386  =  381 bp Oryza sativa (rice) snRNA U3 promoter

OsU3 promoter 6 .. 386  =  381 bp Oryza sativa (rice) snRNA U3 promoter

CaMV 35S promoter 1044 .. 1389  =  346 bp strong constitutive promoter from cauliflower mosaic virus

CaMV 35S promoter 1044 .. 1389  =  346 bp strong constitutive promoter from cauliflower mosaic virus

NOS terminator 5858 .. 6110  =  253 bp nopaline synthase terminator and poly(A) signal

NOS terminator 5858 .. 6110  =  253 bp nopaline synthase terminator and poly(A) signal

pVS1 oriV 9875 .. 10,069  =  195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

pVS1 oriV 9875 .. 10,069  =  195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

CaMV poly(A) signal 12,735 .. 12,909  =  175 bp cauliflower mosaic virus polyadenylation signal

CaMV poly(A) signal 12,735 .. 12,909  =  175 bp cauliflower mosaic virus polyadenylation signal

bom 10,413 .. 10,553  =  141 bp basis of mobility region from pBR322

bom 10,413 .. 10,553  =  141 bp basis of mobility region from pBR322

gRNA scaffold 403 .. 478  =  76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

gRNA scaffold 403 .. 478  =  76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

3xFLAG 1480 .. 1545  =  66 bp 22 amino acids  =  2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site

3xFLAG 1480 .. 1545  =  66 bp 22 amino acids  =  2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site

nucleoplasmin NLS 5698 .. 5745  =  48 bp 16 amino acids  =  1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin

nucleoplasmin NLS 5698 .. 5745  =  48 bp 16 amino acids  =  1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin

attB1 1411 .. 1435  =  25 bp recombination site for the Gateway® BP reaction

attB1 1411 .. 1435  =  25 bp recombination site for the Gateway® BP reaction

attB2 5778 .. 5802  =  25 bp recombination site for the Gateway® BP reaction

attB2 5778 .. 5802  =  25 bp recombination site for the Gateway® BP reaction

RB T-DNA repeat 6355 .. 6379  =  25 bp right border repeat from nopaline C58 T-DNA

RB T-DNA repeat 6355 .. 6379  =  25 bp right border repeat from nopaline C58 T-DNA

LB T-DNA repeat 12,633 .. 12,657  =  25 bp left border repeat from nopaline C58 T-DNA

LB T-DNA repeat 12,633 .. 12,657  =  25 bp left border repeat from nopaline C58 T-DNA

SV40 NLS 1552 .. 1572  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 1552 .. 1572  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

polIII terminator 479 .. 484  =  6 bp RNA polymerase III transcription terminator

polIII terminator 479 .. 484  =  6 bp RNA polymerase III transcription terminator

ATG 1477 .. 1479  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 1477 .. 1479  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

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