Plasmid Files |
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![]() | pRGEB31.dna |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
Cas9 1597 .. 5697 = 4101 bp 1367 amino acids = 158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
Cas9 1597 .. 5697 = 4101 bp 1367 amino acids = 158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
pVS1 RepA 8742 .. 9809 = 1068 bp 355 amino acids = 39.7 kDa Product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
pVS1 RepA 8742 .. 9809 = 1068 bp 355 amino acids = 39.7 kDa Product: replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
HygR 12,949 .. 13,974 = 1026 bp 341 amino acids = 38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
HygR 12,949 .. 13,974 = 1026 bp 341 amino acids = 38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
KanR 11,414 .. 12,208 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
KanR 11,414 .. 12,208 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
CaMV 35S promoter (enhanced) 14,042 .. 14,719 = 678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
CaMV 35S promoter (enhanced) 14,042 .. 14,719 = 678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
pVS1 StaA 7679 .. 8308 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
pVS1 StaA 7679 .. 8308 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
ori 10,739 .. 11,327 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 10,739 .. 11,327 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
OsU3 promoter 6 .. 386 = 381 bp Oryza sativa (rice) snRNA U3 promoter |
OsU3 promoter 6 .. 386 = 381 bp Oryza sativa (rice) snRNA U3 promoter |
CaMV 35S promoter 1044 .. 1389 = 346 bp strong constitutive promoter from cauliflower mosaic virus |
CaMV 35S promoter 1044 .. 1389 = 346 bp strong constitutive promoter from cauliflower mosaic virus |
NOS terminator 5858 .. 6110 = 253 bp nopaline synthase terminator and poly(A) signal |
NOS terminator 5858 .. 6110 = 253 bp nopaline synthase terminator and poly(A) signal |
pVS1 oriV 9875 .. 10,069 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
pVS1 oriV 9875 .. 10,069 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
CaMV poly(A) signal 12,735 .. 12,909 = 175 bp cauliflower mosaic virus polyadenylation signal |
CaMV poly(A) signal 12,735 .. 12,909 = 175 bp cauliflower mosaic virus polyadenylation signal |
bom 10,413 .. 10,553 = 141 bp basis of mobility region from pBR322 |
bom 10,413 .. 10,553 = 141 bp basis of mobility region from pBR322 |
gRNA scaffold 403 .. 478 = 76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
gRNA scaffold 403 .. 478 = 76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
3xFLAG 1480 .. 1545 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
3xFLAG 1480 .. 1545 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
nucleoplasmin NLS 5698 .. 5745 = 48 bp 16 amino acids = 1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin |
nucleoplasmin NLS 5698 .. 5745 = 48 bp 16 amino acids = 1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin |
attB1 1411 .. 1435 = 25 bp recombination site for the Gateway® BP reaction |
attB1 1411 .. 1435 = 25 bp recombination site for the Gateway® BP reaction |
attB2 5778 .. 5802 = 25 bp recombination site for the Gateway® BP reaction |
attB2 5778 .. 5802 = 25 bp recombination site for the Gateway® BP reaction |
RB T-DNA repeat 6355 .. 6379 = 25 bp right border repeat from nopaline C58 T-DNA |
RB T-DNA repeat 6355 .. 6379 = 25 bp right border repeat from nopaline C58 T-DNA |
LB T-DNA repeat 12,633 .. 12,657 = 25 bp left border repeat from nopaline C58 T-DNA |
LB T-DNA repeat 12,633 .. 12,657 = 25 bp left border repeat from nopaline C58 T-DNA |
SV40 NLS 1552 .. 1572 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 1552 .. 1572 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
polIII terminator 479 .. 484 = 6 bp RNA polymerase III transcription terminator |
polIII terminator 479 .. 484 = 6 bp RNA polymerase III transcription terminator |
ATG 1477 .. 1479 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 1477 .. 1479 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ORF: 541 .. 1290 = 750 bp ORF: 249 amino acids = 28.0 kDa |
ORF: 1477 .. 5748 = 4272 bp ORF: 1423 amino acids = 164.6 kDa |
ORF: 6694 .. 7380 = 687 bp ORF: 228 amino acids = 25.1 kDa |
ORF: 12,901 .. 13,641 = 741 bp ORF: 246 amino acids = 27.0 kDa |
ORF: 584 .. 814 = 231 bp ORF: 76 amino acids = 9.1 kDa |
ORF: 7679 .. 8308 = 630 bp ORF: 209 amino acids = 22.1 kDa |
ORF: 8703 .. 9809 = 1107 bp ORF: 368 amino acids = 41.2 kDa |
ORF: 11,547 .. 11,777 = 231 bp ORF: 76 amino acids = 8.3 kDa |
ORF: 7145 .. 7417 = 273 bp ORF: 90 amino acids = 9.9 kDa |
ORF: 8576 .. 8839 = 264 bp ORF: 87 amino acids = 9.1 kDa |
ORF: 11,414 .. 12,208 = 795 bp ORF: 264 amino acids = 31.0 kDa |
ORF: 4 .. 342 = 339 bp ORF: 112 amino acids = 12.7 kDa |
ORF: 1471 .. 4113 = 2643 bp ORF: 880 amino acids = 92.7 kDa |
ORF: 7417 .. 7731 = 315 bp ORF: 104 amino acids = 12.0 kDa |
ORF: 12,949 .. 13,974 = 1026 bp ORF: 341 amino acids = 38.0 kDa |
ORF: 14,146 .. 14,463 = 318 bp ORF: 105 amino acids = 11.6 kDa |
ORF: 14,473 .. 14,931 = 459 bp ORF: 152 amino acids = 16.9 kDa |
ORF: 2943 .. 3236 = 294 bp ORF: 97 amino acids = 10.8 kDa |
ORF: 3507 .. 3956 = 450 bp ORF: 149 amino acids = 17.3 kDa |
ORF: 6726 .. 7076 = 351 bp ORF: 116 amino acids = 12.6 kDa |
ORF: 8583 .. 9176 = 594 bp ORF: 197 amino acids = 22.2 kDa |
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