pRPR1_gRNA_handle_RPR1t

Yeast plasmid for expression of a guide RNA under control of the RNase P RNA (RPR1) promoter and terminator.

Sequence Author: Lu Lab / Addgene #49014

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polIII terminator HindIII (4034) RPR1 promoter pRPR1_gRNA_handle_RPR1t 7693 bp
HindIII  (4034)
1 site
A A G C T T T T C G A A
2μ ori
69 .. 1411  =  1343 bp
yeast 2μ plasmid origin of replication
2μ ori
69 .. 1411  =  1343 bp
yeast 2μ plasmid origin of replication
LEU2
5937 .. 7031  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
LEU2
5937 .. 7031  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required for leucine biosynthesis
yeast auxotrophic marker
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1543 .. 1611  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1612 .. 2403  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2574 .. 3162  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2574 .. 3162  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4765 .. 5220  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4765 .. 5220  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
RPR1 promoter
3626 .. 4032  =  407 bp
promoter for the S. cerevisiae RNase P RNA gene
RPR1 promoter
3626 .. 4032  =  407 bp
promoter for the S. cerevisiae RNase P RNA gene
LEU2 promoter
5520 .. 5924  =  405 bp
LEU2 promoter
5520 .. 5924  =  405 bp
AmpR promoter
1438 .. 1542  =  105 bp
AmpR promoter
1438 .. 1542  =  105 bp
gRNA scaffold
4040 .. 4115  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
4040 .. 4115  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
RPR1 terminator
4128 .. 4190  =  63 bp
transcription terminator for the S. cerevisiae RNase P RNA gene
RPR1 terminator
4128 .. 4190  =  63 bp
transcription terminator for the S. cerevisiae RNase P RNA gene
polIII terminator
4116 .. 4121  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
4116 .. 4121  =  6 bp
RNA polymerase III transcription terminator
ORF:  1543 .. 2403  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4414 .. 4788  =  375 bp
ORF:  124 amino acids  =  14.3 kDa
ORF:  290 .. 610  =  321 bp
ORF:  106 amino acids  =  12.9 kDa
ORF:  5925 .. 7031  =  1107 bp
ORF:  368 amino acids  =  39.4 kDa
ORF:  6599 .. 6910  =  312 bp
ORF:  103 amino acids  =  11.1 kDa
ORF:  1086 .. 1376  =  291 bp
ORF:  96 amino acids  =  11.5 kDa
ORF:  2007 .. 2273  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pRPR1_gRNA_handle_RPR1t.dna file

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