Resources
Plasmid Files

pRPR1_gRNA_handle_RPR1t

Yeast plasmid for expression of a guide RNA under control of the RNase P RNA (RPR1) promoter and terminator.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pRPR1_gRNA_handle_RPR1t Sequence and MappRPR1_gRNA_handle_RPR1t.dna
Map and Sequence File   
Sequence Author:  Lu Lab / Addgene #49014
Download Free Trial Get SnapGene Viewer

 polIII terminator HindIII (4034) RPR1 promoter pRPR1_gRNA_handle_RPR1t 7693 bp
HindIII  (4034)
1 site
A A G C T T T T C G A A
2μ ori
69 .. 1411  =  1343 bp
yeast 2μ plasmid origin of replication
2μ ori
69 .. 1411  =  1343 bp
yeast 2μ plasmid origin of replication
LEU2
5937 .. 7031  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required
for leucine biosynthesis
yeast auxotrophic marker
LEU2
5937 .. 7031  =  1095 bp
364 amino acids  =  39.0 kDa
Product: 3-isopropylmalate dehydrogenase, required
for leucine biosynthesis
yeast auxotrophic marker
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1543 .. 1611  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1612 .. 2403  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1543 .. 2403  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
2574 .. 3162  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2574 .. 3162  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
4765 .. 5220  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
4765 .. 5220  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
RPR1 promoter
3626 .. 4032  =  407 bp
promoter for the S. cerevisiae RNase P RNA gene
RPR1 promoter
3626 .. 4032  =  407 bp
promoter for the S. cerevisiae RNase P RNA gene
LEU2 promoter
5520 .. 5924  =  405 bp
LEU2 promoter
5520 .. 5924  =  405 bp
AmpR promoter
1438 .. 1542  =  105 bp
AmpR promoter
1438 .. 1542  =  105 bp
gRNA scaffold
4040 .. 4115  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
4040 .. 4115  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
RPR1 terminator
4128 .. 4190  =  63 bp
transcription terminator for the S. cerevisiae RNase
P RNA gene
RPR1 terminator
4128 .. 4190  =  63 bp
transcription terminator for the S. cerevisiae RNase
P RNA gene
polIII terminator
4116 .. 4121  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
4116 .. 4121  =  6 bp
RNA polymerase III transcription terminator
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter