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Plasmid Files

pT7-Cas9

In vitro transcription vector with the T7 promoter, for producing myc- and FLAG®-tagged S. pyogenes Cas9.

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pT7-Cas9 Sequence and MappT7-Cas9.dna
Map and Sequence File   
Sequence Author:  OriGene
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 PmeI (5277) SV40 NLS SV40 NLS CMV enhancer pT7-Cas9 9036 bp
PmeI  (5277)
1 site
G T T T A A A C C A A A T T T G
Cas9
1029 .. 5132  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1029 .. 5132  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
NeoR/KanR
7287 .. 8081  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
7287 .. 8081  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
hGH poly(A) signal
5320 .. 5942  =  623 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
5320 .. 5942  =  623 bp
human growth hormone polyadenylation signal
ori
6091 .. 6679  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
6091 .. 6679  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
8606 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
8606 .. 25  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
343 .. 722  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
8116 .. 8473  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
8116 .. 8473  =  358 bp
SV40 enhancer and early promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
723 .. 926  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
AmpR promoter
8475 .. 8579  =  105 bp
AmpR promoter
8475 .. 8579  =  105 bp
HSV TK poly(A) signal
7008 .. 7055  =  48 bp
herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
7008 .. 7055  =  48 bp
herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)
Myc
5202 .. 5231  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
5202 .. 5231  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
FLAG
5250 .. 5273  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
FLAG
5250 .. 5273  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
SV40 NLS
5133 .. 5153  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
5133 .. 5153  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
5160 .. 5180  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
5160 .. 5180  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
952 .. 970  =  19 bp
promoter for bacteriophage T7 RNA polymerase
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