Resources
Plasmid Files

pT7-Guide-IVT

In vitro transcription vector with the T7 promoter, for producing a guide RNA (gRNA).

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pT7-Guide-IVT Sequence and MappT7-Guide-IVT.dna
Map and Sequence File   
Sequence Author:  OriGene
Download Free Trial Get SnapGene Viewer

 BsmBI (3300) BsmBI (3241) EcoRI (3222) EcoRI (3389) pT7-Guide-IVT 3444 bp
BsmBI  (3300)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI  (3241)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
EcoRI  (3222)
2 sites
G A A T T C C T T A A G
EcoRI  (3389)
2 sites
G A A T T C C T T A A G
AmpR
1106 .. 1966  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1106 .. 1174  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1106 .. 1966  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1175 .. 1966  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1106 .. 1966  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
2137 .. 2725  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
2137 .. 2725  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
264 .. 719  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
264 .. 719  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
AmpR promoter
1001 .. 1105  =  105 bp
AmpR promoter
1001 .. 1105  =  105 bp
gRNA scaffold
3300 .. 3375  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
3300 .. 3375  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
T7 promoter
3228 .. 3246  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
3228 .. 3246  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
35 .. 51  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
35 .. 51  =  17 bp
common sequencing primer, one of multiple similar
variants
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter