pX330

Zhang lab plasmid for expressing a chimeric guide RNA (gRNA) together with human codon-optimized Cas9. Also known as pX330-U6-Chimeric_BB-CBh-hSpCas9.

Sequence Author: Zhang Lab / Addgene #42230

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
pol III terminator BbsI (7954) BbsI (7932) nucleoplasmin NLS chicken β-actin promoter ATG pX330 8484 bp
BbsI  (7954)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (7932)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
ATG
432 .. 434  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
432 .. 434  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
435 .. 500  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
435 .. 500  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
507 .. 527  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
507 .. 527  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
552 .. 4652  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
552 .. 4652  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
4653 .. 4700  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
4653 .. 4700  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
AmpR
6007 .. 6867  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6007 .. 6075  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6007 .. 6867  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6076 .. 6867  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6007 .. 6867  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7038 .. 7626  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7038 .. 7626  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5165 .. 5620  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5165 .. 5620  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
8127 .. 8412  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
CMV enhancer
8127 .. 8412  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
chicken β-actin promoter
8414 .. 186  =  257 bp
contains a 19-bp deletion relative to the standard β-actin promoter
chicken β-actin promoter
8414 .. 186  =  257 bp
contains a 19-bp deletion relative to the standard β-actin promoter
U6 promoter
7688 .. 7928  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
7688 .. 7928  =  241 bp
RNA polymerase III promoter for human U6 snRNA
hybrid intron
187 .. 414  =  228 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
hybrid intron
187 .. 414  =  228 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
bGH poly(A) signal
4734 .. 4941  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
4734 .. 4941  =  208 bp
bovine growth hormone polyadenylation signal
AAV2 ITR
4950 .. 5090  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
4950 .. 5090  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
5902 .. 6006  =  105 bp
AmpR promoter
5902 .. 6006  =  105 bp
gRNA scaffold
7955 .. 8030  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
7955 .. 8030  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
pol III terminator
8031 .. 8036  =  6 bp
RNA polymerase III transcription terminator
pol III terminator
8031 .. 8036  =  6 bp
RNA polymerase III transcription terminator
ORF:  28 .. 258  =  231 bp
ORF:  76 amino acids  =  8.2 kDa
ORF:  370 .. 609  =  240 bp
ORF:  79 amino acids  =  9.1 kDa
ORF:  6007 .. 6867  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4964 .. 5188  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  432 .. 4703  =  4272 bp
ORF:  1423 amino acids  =  164.6 kDa
ORF:  8322 .. 164  =  327 bp
ORF:  108 amino acids  =  11.4 kDa
ORF:  4864 .. 5145  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  411 .. 3068  =  2658 bp
ORF:  885 amino acids  =  93.1 kDa
ORF:  6471 .. 6737  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1898 .. 2191  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  2462 .. 2911  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
Click here to try SnapGene

Download pX330.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.