Plasmid Files |
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![]() | pX330A-1x2.dna |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different BpiI sites may not be compatible. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different BpiI sites may not be compatible. |
Cas9 939 .. 5039 = 4101 bp 1367 amino acids = 158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
Cas9 939 .. 5039 = 4101 bp 1367 amino acids = 158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA |
AmpR 6394 .. 7254 = 861 bp 286 amino acids = 31.6 kDa Segment 1: signal sequence 6394 .. 6462 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 6394 .. 7254 = 861 bp 286 amino acids = 31.6 kDa Segment 2: 6463 .. 7254 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 6394 .. 7254 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 7425 .. 8013 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 7425 .. 8013 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 5552 .. 6007 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 5552 .. 6007 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
lacZα 8503 .. 8808 = 306 bp 101 amino acids = 11.2 kDa Product: LacZα fragment of β-galactosidase |
lacZα 8503 .. 8808 = 306 bp 101 amino acids = 11.2 kDa Product: LacZα fragment of β-galactosidase |
CMV enhancer 8 .. 293 = 286 bp human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer |
CMV enhancer 8 .. 293 = 286 bp human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer |
chicken β-actin promoter 295 .. 572 = 278 bp |
chicken β-actin promoter 295 .. 572 = 278 bp |
U6 promoter 8075 .. 8315 = 241 bp RNA polymerase III promoter for human U6 snRNA |
U6 promoter 8075 .. 8315 = 241 bp RNA polymerase III promoter for human U6 snRNA |
hybrid intron 573 .. 801 = 229 bp hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011) |
hybrid intron 573 .. 801 = 229 bp hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011) |
bGH poly(A) signal 5121 .. 5328 = 208 bp bovine growth hormone polyadenylation signal |
bGH poly(A) signal 5121 .. 5328 = 208 bp bovine growth hormone polyadenylation signal |
AAV2 ITR 5337 .. 5477 = 141 bp inverted terminal repeat of adeno-associated virus serotype 2 |
AAV2 ITR 5337 .. 5477 = 141 bp inverted terminal repeat of adeno-associated virus serotype 2 |
AmpR promoter 6289 .. 6393 = 105 bp |
AmpR promoter 6289 .. 6393 = 105 bp |
gRNA scaffold 8342 .. 8417 = 76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
gRNA scaffold 8342 .. 8417 = 76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system |
3xFLAG 822 .. 887 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
3xFLAG 822 .. 887 = 66 bp 22 amino acids = 2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site |
nucleoplasmin NLS 5040 .. 5087 = 48 bp 16 amino acids = 1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin |
nucleoplasmin NLS 5040 .. 5087 = 48 bp 16 amino acids = 1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin |
lac promoter 8852 .. 8882 = 31 bp Segment 3: -10 8852 .. 8858 = 7 bp promoter for the E. coli lac operon |
lac promoter 8852 .. 8882 = 31 bp Segment 2: 8859 .. 8876 = 18 bp promoter for the E. coli lac operon |
lac promoter 8852 .. 8882 = 31 bp Segment 1: -35 8877 .. 8882 = 6 bp promoter for the E. coli lac operon |
lac promoter 8852 .. 8882 = 31 bp 3 segments promoter for the E. coli lac operon |
SV40 NLS 894 .. 914 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
SV40 NLS 894 .. 914 = 21 bp 7 amino acids = 883.1 Da Product: nuclear localization signal of SV40 large T antigen |
lac operator 8828 .. 8844 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
lac operator 8828 .. 8844 = 17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG). |
polIII terminator 8418 .. 8423 = 6 bp RNA polymerase III transcription terminator |
polIII terminator 8418 .. 8423 = 6 bp RNA polymerase III transcription terminator |
ATG 819 .. 821 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ATG 819 .. 821 = 3 bp 1 amino acid = 149.2 Da Product: start codon |
ORF: 757 .. 996 = 240 bp ORF: 79 amino acids = 9.1 kDa |
ORF: 6394 .. 7254 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 203 .. 550 = 348 bp ORF: 115 amino acids = 12.1 kDa |
ORF: 5351 .. 5575 = 225 bp ORF: 74 amino acids = 8.3 kDa |
ORF: 8897 .. 187 = 255 bp ORF: 84 amino acids = 9.2 kDa |
ORF: 393 .. 704 = 312 bp ORF: 103 amino acids = 10.7 kDa |
ORF: 819 .. 5090 = 4272 bp ORF: 1423 amino acids = 164.6 kDa |
ORF: 5251 .. 5532 = 282 bp ORF: 93 amino acids = 10.4 kDa |
ORF: 8503 .. 8808 = 306 bp ORF: 101 amino acids = 11.2 kDa |
ORF: 798 .. 3455 = 2658 bp ORF: 885 amino acids = 93.1 kDa |
ORF: 6858 .. 7124 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 2285 .. 2578 = 294 bp ORF: 97 amino acids = 10.8 kDa |
ORF: 2849 .. 3298 = 450 bp ORF: 149 amino acids = 17.3 kDa |
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