Plasmid Files

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BsaI  (8500) 2 sites

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

BbsI  (8341) 2 sites

Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C.

BpiI  (8341) 2 sites

Sticky ends from different BpiI sites may not be compatible.

BbsI  (8319) 2 sites

Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C.

BpiI  (8319) 2 sites

Sticky ends from different BpiI sites may not be compatible.

BsaI  (8959) 2 sites

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

Cas9 939 .. 5039  =  4101 bp 1367 amino acids  =  158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

Cas9 939 .. 5039  =  4101 bp 1367 amino acids  =  158.3 kDa Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system generates RNA-guided double strand breaks in DNA

AmpR 6394 .. 7254  =  861 bp 286 amino acids  =  31.6 kDa    Segment 1:  signal sequence      6394 .. 6462  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 6394 .. 7254  =  861 bp 286 amino acids  =  31.6 kDa    Segment 2:      6463 .. 7254  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 6394 .. 7254  =  861 bp 286 amino acids  =  31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

ori 7425 .. 8013  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 7425 .. 8013  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori 5552 .. 6007  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori 5552 .. 6007  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

lacZα 8503 .. 8808  =  306 bp 101 amino acids  =  11.2 kDa Product: LacZα fragment of β-galactosidase

lacZα 8503 .. 8808  =  306 bp 101 amino acids  =  11.2 kDa Product: LacZα fragment of β-galactosidase

CMV enhancer 8 .. 293  =  286 bp human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer

CMV enhancer 8 .. 293  =  286 bp human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer

chicken β-actin promoter 295 .. 572  =  278 bp

chicken β-actin promoter 295 .. 572  =  278 bp

U6 promoter 8075 .. 8315  =  241 bp RNA polymerase III promoter for human U6 snRNA

U6 promoter 8075 .. 8315  =  241 bp RNA polymerase III promoter for human U6 snRNA

hybrid intron 573 .. 801  =  229 bp hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)

hybrid intron 573 .. 801  =  229 bp hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)

bGH poly(A) signal 5121 .. 5328  =  208 bp bovine growth hormone polyadenylation signal

bGH poly(A) signal 5121 .. 5328  =  208 bp bovine growth hormone polyadenylation signal

AAV2 ITR 5337 .. 5477  =  141 bp inverted terminal repeat of adeno-associated virus serotype 2

AAV2 ITR 5337 .. 5477  =  141 bp inverted terminal repeat of adeno-associated virus serotype 2

AmpR promoter 6289 .. 6393  =  105 bp

AmpR promoter 6289 .. 6393  =  105 bp

gRNA scaffold 8342 .. 8417  =  76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

gRNA scaffold 8342 .. 8417  =  76 bp guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system

3xFLAG 822 .. 887  =  66 bp 22 amino acids  =  2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site

3xFLAG 822 .. 887  =  66 bp 22 amino acids  =  2.7 kDa Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site

nucleoplasmin NLS 5040 .. 5087  =  48 bp 16 amino acids  =  1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin

nucleoplasmin NLS 5040 .. 5087  =  48 bp 16 amino acids  =  1.7 kDa Product: bipartite nuclear localization signal from nucleoplasmin

lac promoter 8852 .. 8882  =  31 bp    Segment 3:  -10      8852 .. 8858  =  7 bp promoter for the E. coli lac operon

lac promoter 8852 .. 8882  =  31 bp    Segment 2:      8859 .. 8876  =  18 bp promoter for the E. coli lac operon

lac promoter 8852 .. 8882  =  31 bp    Segment 1:  -35      8877 .. 8882  =  6 bp promoter for the E. coli lac operon

lac promoter 8852 .. 8882  =  31 bp 3 segments promoter for the E. coli lac operon

SV40 NLS 894 .. 914  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

SV40 NLS 894 .. 914  =  21 bp 7 amino acids  =  883.1 Da Product: nuclear localization signal of SV40 large T antigen

lac operator 8828 .. 8844  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 8828 .. 8844  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

polIII terminator 8418 .. 8423  =  6 bp RNA polymerase III transcription terminator

polIII terminator 8418 .. 8423  =  6 bp RNA polymerase III transcription terminator

ATG 819 .. 821  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 819 .. 821  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

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