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Plasmid Files

pcDNA-dCas9-VP64

Mammalian cell plasmid for CRISPR activation by expressing catalytically inactive dCas9 fused to the VP64 transcriptional activation domain.

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pcDNA-dCas9-VP64.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Gersbach Lab / Addgene #47107
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SgrDI (9810) PvuI (9259) AhdI (8889) PfoI (7423) RsrII (7164) PflFI - Tth111I (6766) MscI (6730) PstI (6701) PluTI (6651) SfoI (6649) NarI (6648) KasI (6647) SmaI (6461) BmeT110I (6460) AvaI - BsoBI - TspMI - XmaI (6459) AvrII (6438) StuI (6437) BseRI (6434) SexAI * (6205) DraIII (5915) PmeI (5391) AflII (5383) XbaI (5377) BspEI (5337) BsiWI (5327) PacI (5318) AscI (5160) BglII (12) SpeI (249) SnaBI (590) BspDI - ClaI (947) SacII (1005) BsmBI (1201) BmgBI (1228) SwaI (1529) BsgI (1631) AleI (2255) Acc65I (2782) KpnI (2786) PmlI (3301) BstEII (5107)