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Plasmid Files

pcDNA3.1-CibN-dCas9-CibN

Plasmid encoding two copies of CIBN fused to catalytically inactive dCas9, for use in the light-activated CRISPR-Cas9 effector (LACE) system.

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pcDNA3.1-CibN-dCas9-CibN.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Gersbach Lab / Addgene #60553
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PmeI (10,320) AflII (10,312) AscI (9788) PmlI (7929) KpnI (7414) Acc65I (7410) EcoRI (6976) AleI (6883) BsgI (6259) SwaI (6157) BmgBI (5856) SacII (5633) DraIII (212) SexAI * (502) StuI (734) AvrII (735) AvaI - BsoBI - TspMI - XmaI (756) BmeT110I (757) SmaI (758) EagI (851) KasI (944) NarI (945) SfoI (946) PluTI (948) PstI (998) MscI (1027) PflFI - Tth111I (1063) RsrII (1461) PfoI (1720) AhdI (3186) PvuI (3556) SgrDI (4107) BglII (4121) SpeI (4358) SnaBI (4699) BspDI - ClaI (5056)