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pdCas9-humanized

Retroviral vector encoding human codon-optimized catalytically dead dCas9 for CRISPR interference.

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pdCas9-humanized.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qi Lab / Addgene #44246
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SgrAI (10,299) FauNDI - NdeI (9927) BanIII - Bsa29I - BseCI - BshVI - BspDI - BspXI - Bsu15I - BsuTUI - ClaI (6794) EcoT22I - Mph1103I - NsiI - Zsp2I (6793) Cfr42I - KspI - SacII - Sfr303I - SgrBI - SstII (6354) CpoI - CspI - RsrII - Rsr2I (6256) BsiWI - Pfl23II - PspLI (6196) HindIII (6131) Acc36I - BfuAI - BspMI - BveI (6129) AgeI - AsiGI - BshTI - CspAI - PinAI (5730) EcoRI (5610) BglII (1410) Bsp19I - NcoI (1421) AccB7I - PflMI - Van91I (1722) SgrDI (1789) BtgZI (1973) BsaBI * - Bse8I - BseJI * - MamI * (2240) AanI - PsiI (2540) MluI (5256) pdCas9-humanized 10,477 bp
SgrAI  (10,299)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
FauNDI  (9927)
1 site
C A T A T G G T A T A C
NdeI  (9927)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BanIII  (6794)
1 site
A T C G A T T A G C T A
Bsa29I  (6794)
1 site
A T C G A T T A G C T A
BseCI  (6794)
1 site
A T C G A T T A G C T A
BshVI  (6794)
1 site
A T C G A T T A G C T A
BspDI  (6794)
1 site
A T C G A T T A G C T A
BspXI  (6794)
1 site
A T C G A T T A G C T A
Bsu15I  (6794)
1 site
A T C G A T T A G C T A
BsuTUI  (6794)
1 site
A T C G A T T A G C T A
ClaI  (6794)
1 site
A T C G A T T A G C T A
EcoT22I  (6793)
1 site
A T G C A T T A C G T A
Mph1103I  (6793)
1 site
A T G C A T T A C G T A
NsiI  (6793)
1 site
A T G C A T T A C G T A
Zsp2I  (6793)
1 site
A T G C A T T A C G T A
Cfr42I  (6354)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the Cfr42I recognition sequence.
KspI  (6354)
1 site
C C G C G G G G C G C C
SacII  (6354)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
Sfr303I  (6354)
1 site
C C G C G G G G C G C C
SgrBI  (6354)
1 site
C C G C G G G G C G C C
SstII  (6354)
1 site
C C G C G G G G C G C C