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Plasmid Files

pgRNA-bacteria

Plasmid for expressing a single guide RNA (sgRNA) in bacteria.

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pgRNA-bacteria Sequence and MappgRNA-bacteria.dna
Map and Sequence File   
Sequence Author:  Qi Lab / Addgene #44251
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 SpeI (45) pgRNA-bacteria 2584 bp
SpeI  (45)
1 site
A C T A G T T G A T C A
AmpR
1484 .. 2344  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1484 .. 2275  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1484 .. 2344  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2276 .. 2344  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1484 .. 2344  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
725 .. 1313  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
725 .. 1313  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AmpR promoter
2345 .. 2449  =  105 bp
AmpR promoter
2345 .. 2449  =  105 bp
gRNA scaffold
71 .. 146  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
71 .. 146  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
rrnB T1 terminator
455 .. 501  =  47 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
455 .. 501  =  47 bp
transcription terminator T1 from the E. coli rrnB
gene
J23119(SpeI) promoter
16 .. 50  =  35 bp
bacterial promoter (Registry of Standard Biological
Parts BBa_J23119) modified to end with an SpeI
site
J23119(SpeI) promoter
16 .. 50  =  35 bp
bacterial promoter (Registry of Standard Biological
Parts BBa_J23119) modified to end with an SpeI
site
mRFP NT1
51 .. 70  =  20 bp
20-bp sequence complementary to mRFP sequence
mRFP NT1
51 .. 70  =  20 bp
20-bp sequence complementary to mRFP sequence
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