AcGFP1

Aequorea coerulescens green fluorescent protein.

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
No matches
600 400 200 End (720) PflMI (699) BtgI (693) PpuMI - EcoO109I (630) StyI (626) EcoNI (625) TsoI (610) PvuII - MspA1I (534) MflI * - BstYI (500) EciI (493) TaqII (478) BspHI (458) DraIII (450) BssHII (327) PfoI * (281) Bsu36I (269) BtsI - BtsαI (214) Bpu10I (193) BstEII (183) AleI (181) AcuI - Eco57MI (154) BsrBI (92) MscI (74) PluTI (17) SfoI (15) NarI - BsaHI (14) KasI (13) Start (0) AcGFP1 AcGFP1 720 bp
End  (720)
0 sites
PflMI  (699)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BtgI  (693)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
PpuMI  (630)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (630)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
StyI  (626)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EcoNI  (625)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
TsoI  (610)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuII  (534)
1 site
C A G C T G G T C G A C
MspA1I  (534)
1 site
C M G C K G G K C G M C
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
EciI  (493)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
TaqII  (478)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BspHI  (458)
1 site
T C A T G A A G T A C T
DraIII  (450)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BssHII  (327)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
Bsu36I  (269)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BtsI  (214)
1 site
G C A G T G N N C G T C A C
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
Bpu10I  (193)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstEII  (183)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AleI  (181)
1 site
C A C N N N N G T G G T G N N N N C A C
AcuI  (154)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (154)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrBI  (92)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
MscI  (74)
1 site
T G G C C A A C C G G T
PluTI  (17)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (15)
1 site
G G C G C C C C G C G G
NarI  (14)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (14)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (13)
1 site
G G C G C C C C G C G G
Start  (0)
0 sites
AcGFP1
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
Product: Aequorea coerulescens GFP
mammalian codon-optimized
AcGFP1
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
Product: Aequorea coerulescens GFP
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  3 .. 272  =  270 bp
ORF:  89 amino acids  =  10.5 kDa  (no start codon)
ORF:  217 .. 720  =  504 bp
ORF:  167 amino acids  =  17.0 kDa  (no start codon)
Click here to try SnapGene

Download AcGFP1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.