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Plasmid Files

Azami-Green

Green fluorescent protein from Galaxeidae coral.

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Azami-Green Sequence and MapAzami-Green.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 Azami-Green End (681) DraIII (573) BspDI - ClaI (510) MscI (503) EaeI (501) HincII - HpaI (474) BsiWI - SnaBI (444) BsaWI (390) MslI (325) BmrI (276) Bsu36I (254) EcoP15I (248) PspFI (220) BseYI (216) EcoRV (168) DrdI (130) BsaBI * (28) Start (0) Azami-Green 681 bp
End  (681)
0 sites
DraIII  (573)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BspDI  (510)
1 site
A T C G A T T A G C T A
ClaI  (510)
1 site
A T C G A T T A G C T A
MscI  (503)
1 site
T G G C C A A C C G G T
EaeI  (501)
1 site
Y G G C C R R C C G G Y
HincII  (474)
1 site
G T Y R A C C A R Y T G
HpaI  (474)
1 site
G T T A A C C A A T T G
BsiWI  (444)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (444)
1 site
T A C G T A A T G C A T
BsaWI  (390)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmrI  (276)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EcoP15I  (248)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
PspFI  (220)
1 site
C C C A G C G G G T C G
BseYI  (216)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
EcoRV  (168)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BsaBI  (28)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
Start  (0)
0 sites
Azami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.1 kDa
Product: Azami-Green fluorescent protein (Karasawa
et al., 2003)
Azami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.1 kDa
Product: Azami-Green fluorescent protein (Karasawa
et al., 2003)
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