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Plasmid Files

Dendra2

Monomeric photoswitchable green-to-red fluorescent protein from Dendronephthya.

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Dendra2 Sequence and MapDendra2.dna
Map and Sequence File   
Sequence Author:  Clontech
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 600 400 200 End (693) BtgI (653) BstYI (602) PflMI (585) BstXI (558) BsaAI - PmlI (456) BanII (437) BbsI (424) BglI (344) AleI (193) HaeII - PluTI (158) SfoI (156) NarI (155) KasI (154) TaqII (139) NspI (39) EcoNI (25) BclI * (23) SmaI (11) TspMI - XmaI (9) Start (0) Dendra2 693 bp
End  (693)
0 sites
BtgI  (653)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BstYI  (602)
1 site
R G A T C Y Y C T A G R
PflMI  (585)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BstXI  (558)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsaAI  (456)
1 site
Y A C G T R R T G C A Y
PmlI  (456)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BanII  (437)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglI  (344)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AleI  (193)
1 site
C A C N N N N G T G G T G N N N N C A C
HaeII  (158)
1 site
R G C G C Y Y C G C G R
PluTI  (158)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (156)
1 site
G G C G C C C C G C G G
NarI  (155)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (154)
1 site
G G C G C C C C G C G G
TaqII  (139)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
NspI  (39)
1 site
R C A T G Y Y G T A C R
EcoNI  (25)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SmaI  (11)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (9)
1 site
C C C G G G G G G C C C
XmaI  (9)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
Start  (0)
0 sites
Dendra2
1 .. 693  =  693 bp
230 amino acids  =  26.1 kDa
Product: monomeric photoswitchable green-to-red
fluorescent protein from Dendronephthya
mammalian codon-optimized
Dendra2
1 .. 693  =  693 bp
230 amino acids  =  26.1 kDa
Product: monomeric photoswitchable green-to-red
fluorescent protein from Dendronephthya
mammalian codon-optimized
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