Dronpa-Green1

Monomeric photoswitchable Dronpa-Green1 fluorescent protein.

Sequence Author: MBL International

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No matches
600 400 200 End (678) BsmI (648) BsiHKAI - Bsp1286I (638) DraIII (573) PsiI (533) MscI (503) EaeI (501) AcuI - Eco57MI (481) HpaI - HincII (474) EarI (397) ApoI (353) BbsI (296) Bsu36I (254) AccI (230) EciI (158) DrdI (130) XmnI (100) StyI (90) NruI (73) HaeII (53) Start (0) Dronpa-Green1 Dronpa-Green1 678 bp
End  (678)
0 sites
BsmI  (648)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsiHKAI  (638)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (638)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
DraIII  (573)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (533)
1 site
T T A T A A A A T A T T
MscI  (503)
1 site
T G G C C A A C C G G T
EaeI  (501)
1 site
Y G G C C R R C C G G Y
AcuI  (481)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (481)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
HpaI  (474)
1 site
G T T A A C C A A T T G
HincII  (474)
1 site
G T Y R A C C A R Y T G
EarI  (397)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
ApoI  (353)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BbsI  (296)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AccI  (230)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
EciI  (158)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
XmnI  (100)
1 site
G A A N N N N T T C C T T N N N N A A G
StyI  (90)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NruI  (73)
1 site
T C G C G A A G C G C T
HaeII  (53)
1 site
R G C G C Y Y C G C G R
Start  (0)
0 sites
Dronpa-Green1
1 .. 678  =  678 bp
225 amino acids  =  25.6 kDa
Product: monomeric photoswitchable Dronpa-Green1 fluorescent protein
Dronpa-Green1
1 .. 678  =  678 bp
225 amino acids  =  25.6 kDa
Product: monomeric photoswitchable Dronpa-Green1 fluorescent protein
ORF:  1 .. 678  =  678 bp
ORF:  225 amino acids  =  25.6 kDa
ORF:  1 .. 678  =  678 bp
ORF:  226 amino acids  =  26.4 kDa  (no start codon)
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Download Dronpa-Green1.dna file

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Individual Sequences & Maps

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