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Plasmid Files

Dronpa-Green3

Photoswitchable Dronpa-Green3 fluorescent protein.

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Dronpa-Green3 Sequence and MapDronpa-Green3.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 Dronpa-Green3 End (678) BsmI (648) BsiHKAI - Bsp1286I (638) DraIII (573) PsiI (533) MscI (503) EaeI (501) AcuI - BsrBI - Eco57MI (481) EarI (397) ApoI (353) BbsI (296) Bsu36I (254) AccI (230) EciI (158) DrdI (130) XmnI (100) StyI (90) NruI (73) HaeII (53) Start (0) Dronpa-Green3 678 bp
End  (678)
0 sites
BsmI  (648)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsiHKAI  (638)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (638)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
DraIII  (573)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (533)
1 site
T T A T A A A A T A T T
MscI  (503)
1 site
T G G C C A A C C G G T
EaeI  (501)
1 site
Y G G C C R R C C G G Y
AcuI  (481)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrBI  (481)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
Eco57MI  (481)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (397)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
ApoI  (353)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BbsI  (296)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AccI  (230)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
EciI  (158)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
XmnI  (100)
1 site
G A A N N N N T T C C T T N N N N A A G
StyI  (90)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NruI  (73)
1 site
T C G C G A A G C G C T
HaeII  (53)
1 site
R G C G C Y Y C G C G R
Start  (0)
0 sites
Dronpa-Green3
1 .. 678  =  678 bp
225 amino acids  =  25.5 kDa
Product: photoswitchable Dronpa-Green3
fluorescent protein
Dronpa-Green3
1 .. 678  =  678 bp
225 amino acids  =  25.5 kDa
Product: photoswitchable Dronpa-Green3
fluorescent protein
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