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Plasmid Files

DsRed-Monomer

Monomeric derivative of DsRed fluorescent protein.

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DsRed-Monomer Sequence and MapDsRed-Monomer.dna
Map and Sequence File   
Sequence Author:  Clontech
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 600 400 200 End (678) NmeAIII (674) BglI (665) BsiHKAI (647) AleI - MslI (628) BtgI (626) DraIII - PflMI (582) MspA1I - PvuII (564) BsrGI - TatI (539) BglII - BstYI (479) PspFI (429) BseYI (425) NaeI (423) BbsI - BsrFI - NgoMIV (421) EcoP15I (352) SbfI (341) BseRI (321) HaeII (285) AfeI (283) BmeT110I (263) AvaI - BsoBI (262) StuI (211) AhdI (180) BstEII (141) TaqII (136) BaeGI - Bme1580I (121) FspI - FspAI (49) AatII (20) ZraI (18) BsaHI (17) Start (0) DsRed-Monomer 678 bp
End  (678)
0 sites
NmeAIII  (674)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (665)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsiHKAI  (647)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
AleI  (628)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (628)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BtgI  (626)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
DraIII  (582)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PflMI  (582)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
MspA1I  (564)
1 site
C M G C K G G K C G M C
PvuII  (564)
1 site
C A G C T G G T C G A C
BsrGI  (539)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (539)
1 site
W G T A C W W C A T G W
BglII  (479)
1 site
A G A T C T T C T A G A
BstYI  (479)
1 site
R G A T C Y Y C T A G R
PspFI  (429)
1 site
C C C A G C G G G T C G
BseYI  (425)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
NaeI  (423)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrFI  (421)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
NgoMIV  (421)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
EcoP15I  (352)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
SbfI  (341)
1 site
C C T G C A G G G G A C G T C C
BseRI  (321)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
HaeII  (285)
1 site
R G C G C Y Y C G C G R
AfeI  (283)
1 site
A G C G C T T C G C G A
BmeT110I  (263)
1 site
C Y C G R G G R G C Y C
AvaI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
StuI  (211)
1 site
A G G C C T T C C G G A
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (141)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
TaqII  (136)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BaeGI  (121)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (121)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
FspI  (49)
1 site
T G C G C A A C G C G T
FspAI  (49)
1 site
R T G C G C A Y Y A C G C G T R
AatII  (20)
1 site
G A C G T C C T G C A G
ZraI  (18)
1 site
G A C G T C C T G C A G
BsaHI  (17)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
Start  (0)
0 sites
DsRed-Monomer
1 .. 678  =  678 bp
225 amino acids  =  25.4 kDa
Product: monomeric derivative of DsRed fluorescent
protein (Strongin et al., 2007)
mammalian codon-optimized
DsRed-Monomer
1 .. 678  =  678 bp
225 amino acids  =  25.4 kDa
Product: monomeric derivative of DsRed fluorescent
protein (Strongin et al., 2007)
mammalian codon-optimized
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