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Plasmid Files

E2-Red/Green

Noncytotoxic tetrameric dual-color red-and-green variant of DsRed fluorescent protein.

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E2-Red_Green.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Glick Lab
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600 400 200 End (678) AleI - OliI (628) BstDSI - BtgI (626) BalI - MlsI - MluNI - MscI - Msp20I (547) AccB7I * - PflMI * - Van91I * (519) CsiI - MabI - SexAI * (518) BstX2I - BstYI - MflI * - PsuI - XhoII (479) GsaI - PspFI (429) BseYI (425) BbsI - BpiI - BpuAI - BstV2I (421) MmeI (416) AcvI - BbrPI - Eco72I - PmaCI - PmlI - PspCI (363) SbfI - SdaI - Sse8387I (341) BseRI (321) BmeT110I (263) Ama87I - AvaI - BsiHKCI - BsoBI - Eco88I (262) BfiI - BmrI - BmuI (183) AhdI - AspEI - BmeRI - DriI - Eam1105I (180) TaqII (136) AarI - Acc36I - BfuAI - BspMI - BveI (129) BauI - BssS α I - Bst2BI (73) Alw21I - Bbv12I - BsiHKAI (50) Alw44I - ApaLI - VneI (46) Start (0) E2-Red/Green 678 bp
End  (678)
0 sites
AleI  (628)
1 site
C A C N N N N G T G G T G N N N N C A C
OliI  (628)
1 site
C A C N N N N G T G G T G N N N N C A C
BstDSI  (626)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BstDSI sites may not be compatible.
BtgI  (626)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BalI  (547)
1 site
T G G C C A A C C G G T
MlsI  (547)
1 site
T G G C C A A C C G G T
MluNI  (547)
1 site
T G G C C A A C C G G T
MscI  (547)
1 site
T G G C C A A C C G G T
Msp20I  (547)
1 site
T G G C C A A C C G G T
AccB7I  (519)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different AccB7I sites may not be compatible.
PflMI  (519)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
Van91I  (519)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different Van91I sites may not be compatible.
CsiI  (518)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
MabI  (518)
1 site
A C C W G G T T G G W C C A

Sticky ends from different MabI sites may not be compatible.
SexAI  (518)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BstX2I  (479)
1 site
R G A T C Y Y C T A G R
BstYI  (479)
1 site
R G A T C Y Y C T A G R
MflI  (479)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
PsuI  (479)
1 site
R G A T C Y Y C T A G R
XhoII  (479)
1 site
R G A T C Y Y C T A G R
GsaI  (429)
1 site
C C C A G C G G G T C G
PspFI  (429)
1 site
C C C A G C G G G T C G
BseYI  (425)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BpuAI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpuAI sites may not be compatible.
BstV2I  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BstV2I sites may not be compatible.
MmeI  (416)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AcvI  (363)
1 site
C A C G T G G T G C A C
BbrPI  (363)
1 site
C A C G T G G T G C A C
Eco72I  (363)
1 site
C A C G T G G T G C A C
PmaCI  (363)
1 site
C A C G T G G T G C A C
PmlI  (363)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
PspCI  (363)
1 site
C A C G T G G T G C A C
SbfI  (341)
1 site
C C T G C A G G G G A C G T C C
SdaI  (341)
1 site
C C T G C A G G G G A C G T C C
Sse8387I  (341)
1 site
C C T G C A G G G G A C G T C C
BseRI  (321)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BmeT110I  (263)
1 site
C Y C G R G G R G C Y C
Ama87I  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different Ama87I sites may not be compatible.
AvaI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsiHKCI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsiHKCI sites may not be compatible.
BsoBI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
Eco88I  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different Eco88I sites may not be compatible.
BfiI  (183)
1 site
A C T G G G ( N ) 4 ( N ) 1-3 T G A C C C ( N ) 4

Efficient cleavage requires at least two copies of the BfiI recognition sequence.
Sticky ends from different BfiI sites may not be compatible.
Unlike most restriction enzymes, BfiI can cleave DNA in the absence of magnesium.
BmrI  (183)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BmuI  (183)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmuI may be hard to ligate.
Sticky ends from different BmuI sites may not be compatible.
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AspEI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AspEI may be hard to ligate.
Sticky ends from different AspEI sites may not be compatible.
BmeRI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by BmeRI may be hard to ligate.
Sticky ends from different BmeRI sites may not be compatible.
DriI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by DriI may be hard to ligate.
Sticky ends from different DriI sites may not be compatible.
Eam1105I  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by Eam1105I may be hard to ligate.
Sticky ends from different Eam1105I sites may not be compatible.
TaqII  (136)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
AarI  (129)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
Acc36I  (129)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Sticky ends from different Acc36I sites may not be compatible.
BfuAI  (129)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (129)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BveI  (129)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BveI recognition sequence.
Sticky ends from different BveI sites may not be compatible.
After cleavage, BveI can remain bound to DNA and alter its electrophoretic mobility.
BauI  (73)
1 site
C A C G A G G T G C T C
BssSαI  (73)
1 site
C A C G A G G T G C T C
Bst2BI  (73)
1 site
C A C G A G G T G C T C
Alw21I  (50)
1 site
G W G C W C C W C G W G

Sticky ends from different Alw21I sites may not be compatible.
Bbv12I  (50)
1 site
G W G C W C C W C G W G

Sticky ends from different Bbv12I sites may not be compatible.
BsiHKAI  (50)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Alw44I  (46)
1 site
G T G C A C C A C G T G
ApaLI  (46)
1 site
G T G C A C C A C G T G
VneI  (46)
1 site
G T G C A C C A C G T G
Start  (0)
0 sites
E2-Red/Green
1 .. 678  =  678 bp
225 amino acids  =  25.8 kDa
Product: noncytotoxic tetrameric dual red-and-green variant of DsRed fluorescent protein (Strack et al., 2009)
mammalian codon-optimized
E2-Red/Green
1 .. 678  =  678 bp
225 amino acids  =  25.8 kDa
Product: noncytotoxic tetrameric dual red-and-green variant of DsRed fluorescent protein (Strack et al., 2009)
mammalian codon-optimized
ORF:  1 .. 678  =  678 bp
ORF:  225 amino acids  =  25.8 kDa
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