EosFP

Green-to-red photoswitchable fluorescent protein.
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No matches
600 400 200 End (681) NspI - SphI (640) PflMI (573) Bpu10I (536) AcuI - Eco57MI (501) BtgI - StyI - NcoI (473) BanII (422) MslI (322) BsrDI (302) BstBI (284) HindIII (273) Bsu36I (251) BsmI (178) EcoRV (165) EciI (155) MflI * - BstYI (120) BaeGI - Bme1580I (62) BanI (59) Start (0) EosFP EosFP 681 bp
End  (681)
0 sites
NspI  (640)
1 site
R C A T G Y Y G T A C R
SphI  (640)
1 site
G C A T G C C G T A C G
PflMI  (573)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Bpu10I  (536)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AcuI  (501)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (501)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgI  (473)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
StyI  (473)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (473)
1 site
C C A T G G G G T A C C
BanII  (422)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
MslI  (322)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsrDI  (302)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BstBI  (284)
1 site
T T C G A A A A G C T T
HindIII  (273)
1 site
A A G C T T T T C G A A
Bsu36I  (251)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsmI  (178)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
EcoRV  (165)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EciI  (155)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
MflI  (120)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (120)
1 site
R G A T C Y Y C T A G R
BaeGI  (62)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (62)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BanI  (59)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
Start  (0)
0 sites
EosFP
1 .. 681  =  681 bp
226 amino acids  =  25.8 kDa
Product: green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)
EosFP
1 .. 681  =  681 bp
226 amino acids  =  25.8 kDa
Product: green-to-red photoswitchable fluorescent protein (Wiedenmann et al., 2004)
ORF:  1 .. 681  =  681 bp
ORF:  226 amino acids  =  25.8 kDa
ORF:  1 .. 504  =  504 bp
ORF:  168 amino acids  =  19.3 kDa
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Download EosFP.dna file

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Individual Sequences & Maps

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