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Plasmid Files

HcRed1

Far-red fluorescent variant of Heteractis crispa chromoprotein.

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HcRed1 Sequence and MapHcRed1.dna
Map and Sequence File   
Sequence Author:  Clontech
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 600 400 200 End (687) BfuAI - BspMI (674) SfiI (649) ScaI (625) AlwNI (603) BssHII (552) BsaWI (533) EagI (468) AleI (436) XcmI (435) BanII (428) NmeAIII (248) BstYI (227) EcoO109I - PpuMI (203) HaeII - PluTI (149) SfoI (147) BsaHI - NarI (146) KasI (145) BsaAI - PmlI (135) NaeI (111) NgoMIV (109) BsrGI (41) FspI - FspAI (31) EcoNI (16) Start (0) HcRed1 687 bp
End  (687)
0 sites
BfuAI  (674)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (674)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SfiI  (649)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
ScaI  (625)
1 site
A G T A C T T C A T G A
AlwNI  (603)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssHII  (552)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BsaWI  (533)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
EagI  (468)
1 site
C G G C C G G C C G G C
AleI  (436)
1 site
C A C N N N N G T G G T G N N N N C A C
XcmI  (435)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BanII  (428)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
NmeAIII  (248)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstYI  (227)
1 site
R G A T C Y Y C T A G R
EcoO109I  (203)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (203)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
HaeII  (149)
1 site
R G C G C Y Y C G C G R
PluTI  (149)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (147)
1 site
G G C G C C C C G C G G
BsaHI  (146)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (146)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (145)
1 site
G G C G C C C C G C G G
BsaAI  (135)
1 site
Y A C G T R R T G C A Y
PmlI  (135)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
NaeI  (111)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (109)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BsrGI  (41)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
FspI  (31)
1 site
T G C G C A A C G C G T
FspAI  (31)
1 site
R T G C G C A Y Y A C G C G T R
EcoNI  (16)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Start  (0)
0 sites
HcRed1
1 .. 687  =  687 bp
228 amino acids  =  25.8 kDa
Product: far-red fluorescent variant of Heteractis
crispa
chromoprotein
mammalian codon-optimized
HcRed1
1 .. 687  =  687 bp
228 amino acids  =  25.8 kDa
Product: far-red fluorescent variant of Heteractis
crispa
chromoprotein
mammalian codon-optimized
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