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Plasmid Files

KikGR1

Photoconvertible Kikume green-to-red fluorescent protein.

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KikGR1 Sequence and MapKikGR1.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (687) StyI (670) BsiHKAI (647) DraIII (582) AlwNI (347) Bsu36I (254) AccI (230) BsmI (181) EcoRV (168) EciI (158) EarI (155) DrdI (130) BaeGI - Bme1580I (65) HaeII (53) Start (0) KikGR1 687 bp
End  (687)
0 sites
StyI  (670)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsiHKAI  (647)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
DraIII  (582)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AlwNI  (347)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AccI  (230)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsmI  (181)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
EcoRV  (168)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
EciI  (158)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
EarI  (155)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BaeGI  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
HaeII  (53)
1 site
R G C G C Y Y C G C G R
Start  (0)
0 sites
KikGR1
1 .. 687  =  687 bp
228 amino acids  =  25.9 kDa
Product: photoconvertible Kikume green-to-red
fluorescent protein (Tsutsui et al., 2005)
KikGR1
1 .. 687  =  687 bp
228 amino acids  =  25.9 kDa
Product: photoconvertible Kikume green-to-red
fluorescent protein (Tsutsui et al., 2005)
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