KillerOrange

Orange fluorescent protein KillerOrange, a genetically-encoded photosensitizer derived from KillerRed.
|Download SnapGene Viewer
No matches
600 400 200 End (714) HaeII (692) PmlI - BsaAI (639) MflI * - BstYI (569) NspI (447) PciI (443) PspFI (434) BseYI (430) BfuAI - PaqCI - BspMI (352) BlpI (334) BanI (304) BsiEI (279) Bpu10I (268) EcoO109I (264) AvaI - BsoBI (259) BanII (206) BsrFI (148) BsiHKAI (134) ApaLI (130) EcoP15I (129) Start (0) KillerOrange KillerOrange 714 bp
End  (714)
0 sites
HaeII  (692)
1 site
R G C G C Y Y C G C G R
PmlI  (639)
1 site
C A C G T G G T G C A C
BsaAI  (639)
1 site
Y A C G T R R T G C A Y
MflI  (569)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (569)
1 site
R G A T C Y Y C T A G R
NspI  (447)
1 site
R C A T G Y Y G T A C R
PciI  (443)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (434)
1 site
C C C A G C G G G T C G
BseYI  (430)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (352)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
PaqCI  (352)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BspMI  (352)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (334)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BanI  (304)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BsiEI  (279)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
Bpu10I  (268)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (264)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AvaI  (259)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (259)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BanII  (206)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsrFI  (148)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsiHKAI  (134)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (130)
1 site
G T G C A C C A C G T G
EcoP15I  (129)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
Start  (0)
0 sites
KillerOrange
1 .. 714  =  714 bp
237 amino acids  =  26.4 kDa
Product: orange fluorescent protein KillerOrange, a genetically-encoded photosensitizer derived from KillerRed (Sarkisyan et al., 2015)
mammalian codon-optimized
KillerOrange
1 .. 714  =  714 bp
237 amino acids  =  26.4 kDa
Product: orange fluorescent protein KillerOrange, a genetically-encoded photosensitizer derived from KillerRed (Sarkisyan et al., 2015)
mammalian codon-optimized
ORF:  1 .. 714  =  714 bp
ORF:  237 amino acids  =  26.4 kDa
ORF:  3 .. 701  =  699 bp
ORF:  232 amino acids  =  27.8 kDa  (no start codon)
ORF:  1 .. 714  =  714 bp
ORF:  238 amino acids  =  22.9 kDa  (no start codon)
ORF:  327 .. 713  =  387 bp
ORF:  128 amino acids  =  14.4 kDa  (no start codon)
Click here to try SnapGene

Download KillerOrange.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.