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Plasmid Files

Kohinoor

Fast-switching photoswitchable fluorescent protein useful for fluorescence nanoscopy.

 
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Kohinoor.dna
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600 400 200 Kohinoor End (675) BsaMI - BsmI - Mva1269I - PctI (645) BbuI - BstNSI - NspI - PaeI - SphI - XceI (637) MslI - RseI - SmiMI (632) AdeI - DraIII (570) AanI - PsiI (530) BalI - MlsI - MluNI - MscI - Msp20I (500) AcoI - CfrI - EaeI (498) BtgZI (479) AcuI - Eco57I - Eco57MI (478) BspQI - LguI - PciSI - SapI (454) AcsI - ApoI - XapI (350) BbsI - BpiI - BpuAI - BstV2I (293) AxyI - Bse21I - Bsu36I - Eco81I (251) EciI (155) AasI - DrdI - DseDI (127) Asp700I - MroXI - PdmI - XmnI (97) BssT1I - Eco130I - EcoT14I - ErhI - StyI (87) BfoI - BstH2I - HaeII (50) Start (0) Kohinoor 675 bp
End  (675)
0 sites
BsaMI  (645)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsaMI sites may not be compatible.
BsmI  (645)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
Mva1269I  (645)
1 site
G A A T G C N C T T A C G N

Sticky ends from different Mva1269I sites may not be compatible.
PctI  (645)
1 site
G A A T G C N C T T A C G N

Sticky ends from different PctI sites may not be compatible.
BbuI  (637)
1 site
G C A T G C C G T A C G
BstNSI  (637)
1 site
R C A T G Y Y G T A C R
NspI  (637)
1 site
R C A T G Y Y G T A C R
PaeI  (637)
1 site
G C A T G C C G T A C G
SphI  (637)
1 site
G C A T G C C G T A C G
XceI  (637)
1 site
R C A T G Y Y G T A C R
MslI  (632)
1 site
C A Y N N N N R T G G T R N N N N Y A C
RseI  (632)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SmiMI  (632)
1 site
C A Y N N N N R T G G T R N N N N Y A C
AdeI  (570)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different AdeI sites may not be compatible.
DraIII  (570)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AanI  (530)
1 site
T T A T A A A A T A T T
PsiI  (530)
1 site
T T A T A A A A T A T T
BalI  (500)
1 site
T G G C C A A C C G G T
MlsI  (500)
1 site
T G G C C A A C C G G T
MluNI  (500)
1 site
T G G C C A A C C G G T
MscI  (500)
1 site
T G G C C A A C C G G T
Msp20I  (500)
1 site
T G G C C A A C C G G T
AcoI  (498)
1 site
Y G G C C R R C C G G Y
CfrI  (498)
1 site
Y G G C C R R C C G G Y
EaeI  (498)
1 site
Y G G C C R R C C G G Y
BtgZI  (479)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AcuI  (478)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57I  (478)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the Eco57I recognition sequence.
Sticky ends from different Eco57I sites may not be compatible.
After cleavage, Eco57I can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (478)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BspQI  (454)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
LguI  (454)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different LguI sites may not be compatible.
PciSI  (454)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different PciSI sites may not be compatible.
SapI  (454)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AcsI  (350)
1 site
R A A T T Y Y T T A A R
ApoI  (350)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
XapI  (350)
1 site
R A A T T Y Y T T A A R
BbsI  (293)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (293)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BpuAI  (293)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpuAI sites may not be compatible.
BstV2I  (293)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BstV2I sites may not be compatible.
AxyI  (251)
1 site
C C T N A G G G G A N T C C

Sticky ends from different AxyI sites may not be compatible.
Bse21I  (251)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bse21I sites may not be compatible.
Bsu36I  (251)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
Eco81I  (251)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Eco81I sites may not be compatible.
EciI  (155)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
AasI  (127)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different AasI sites may not be compatible.
DrdI  (127)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
DseDI  (127)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DseDI sites may not be compatible.
Asp700I  (97)
1 site
G A A N N N N T T C C T T N N N N A A G
MroXI  (97)
1 site
G A A N N N N T T C C T T N N N N A A G
PdmI  (97)
1 site
G A A N N N N T T C C T T N N N N A A G
XmnI  (97)
1 site
G A A N N N N T T C C T T N N N N A A G
BssT1I  (87)
1 site
C C W W G G G G W W C C

Sticky ends from different BssT1I sites may not be compatible.
Eco130I  (87)
1 site
C C W W G G G G W W C C

Sticky ends from different Eco130I sites may not be compatible.
EcoT14I  (87)
1 site
C C W W G G G G W W C C

Sticky ends from different EcoT14I sites may not be compatible.
ErhI  (87)
1 site
C C W W G G G G W W C C

Sticky ends from different ErhI sites may not be compatible.
StyI  (87)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BfoI  (50)
1 site
R G C G C Y Y C G C G R
BstH2I  (50)
1 site
R G C G C Y Y C G C G R
HaeII  (50)
1 site
R G C G C Y Y C G C G R
Start  (0)
0 sites
Kohinoor
1 .. 675  =  675 bp
224 amino acids  =  25.3 kDa
Product: fast-switching photoswitchable fluorescent protein useful for fluorescence nanoscopy (Tiwari et al., 2015)
Kohinoor
1 .. 675  =  675 bp
224 amino acids  =  25.3 kDa
Product: fast-switching photoswitchable fluorescent protein useful for fluorescence nanoscopy (Tiwari et al., 2015)
ORF:  1 .. 675  =  675 bp
ORF:  224 amino acids  =  25.3 kDa
ORF:  1 .. 675  =  675 bp
ORF:  225 amino acids  =  26.0 kDa  (no start codon)
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