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Plasmid Files

Kusabira-Orange

Kusabira-Orange fluorescent protein.

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Kusabira-Orange Sequence and MapKusabira-Orange.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (657) BsrDI (648) BtgZI (574) HindIII (493) BfuAI - BspMI (460) HincII - HpaI (378) ApoI (364) BsiHKAI - Bsp1286I (356) FspI - FspAI (323) BstAPI (319) PstI (315) SfcI (311) BstBI (293) StuI (266) EarI (221) AhdI (177) StyI (143) MscI (142) MluI (129) DrdI (52) ScaI - XmnI (37) TatI (35) Start (0) Kusabira-Orange 657 bp
End  (657)
0 sites
BsrDI  (648)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BtgZI  (574)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
HindIII  (493)
1 site
A A G C T T T T C G A A
BfuAI  (460)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (460)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
HincII  (378)
1 site
G T Y R A C C A R Y T G
HpaI  (378)
1 site
G T T A A C C A A T T G
ApoI  (364)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BsiHKAI  (356)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (356)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
FspI  (323)
1 site
T G C G C A A C G C G T
FspAI  (323)
1 site
R T G C G C A Y Y A C G C G T R
BstAPI  (319)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PstI  (315)
1 site
C T G C A G G A C G T C
SfcI  (311)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BstBI  (293)
1 site
T T C G A A A A G C T T
StuI  (266)
1 site
A G G C C T T C C G G A
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (143)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (142)
1 site
T G G C C A A C C G G T
MluI  (129)
1 site
A C G C G T T G C G C A
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
ScaI  (37)
1 site
A G T A C T T C A T G A
XmnI  (37)
1 site
G A A N N N N T T C C T T N N N N A A G
TatI  (35)
1 site
W G T A C W W C A T G W
Start  (0)
0 sites
Kusabira-Orange
1 .. 657  =  657 bp
218 amino acids  =  24.4 kDa
Product: Kusabira-Orange fluorescent protein
(Karasawa et al., 2004)
Kusabira-Orange
1 .. 657  =  657 bp
218 amino acids  =  24.4 kDa
Product: Kusabira-Orange fluorescent protein
(Karasawa et al., 2004)
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