LSSmOrange

Monomeric orange fluorescent protein with a large Stokes shift, derived from mOrange.

Sequence Author: Verkhusha Lab

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No matches
600 400 200 End (711) TatI - BsrGI (701) NmeAIII (689) XcmI (687) BsrFI - SgrAI (682) AleI (643) HincII (600) AccI (599) SalI (598) BsgI (595) Bpu10I (502) BsrBI (459) BstXI (441) BbsI (436) BtgI - NcoI (434) PstI - SbfI (356) SfcI (352) SmlI (262) BpuEI (247) BsaAI (231) AhdI (195) BssSI - BssSαI (88) BanII - Bsp1286I (76) FspI - FspAI (64) MscI (31) Start (0) LSSmOrange LSSmOrange 711 bp
End  (711)
0 sites
TatI  (701)
1 site
W G T A C W W C A T G W
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NmeAIII  (689)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XcmI  (687)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (682)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (682)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AleI  (643)
1 site
C A C N N N N G T G G T G N N N N C A C
HincII  (600)
1 site
G T Y R A C C A R Y T G
AccI  (599)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (598)
1 site
G T C G A C C A G C T G
BsgI  (595)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
Bpu10I  (502)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsrBI  (459)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
BstXI  (441)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BbsI  (436)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgI  (434)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (434)
1 site
C C A T G G G G T A C C
PstI  (356)
1 site
C T G C A G G A C G T C
SbfI  (356)
1 site
C C T G C A G G G G A C G T C C
SfcI  (352)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SmlI  (262)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (247)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (231)
1 site
Y A C G T R R T G C A Y
AhdI  (195)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BssSI  (88)
1 site
C A C G A G G T G C T C
BssSαI  (88)
1 site
C A C G A G G T G C T C
BanII  (76)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (76)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
FspI  (64)
1 site
T G C G C A A C G C G T
FspAI  (64)
1 site
R T G C G C A Y Y A C G C G T R
MscI  (31)
1 site
T G G C C A A C C G G T
Start  (0)
0 sites
LSSmOrange
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: monomeric orange fluorescent protein with a large Stokes shift (Shcherbakova et al., 2012)
mammalian codon-optimized
LSSmOrange
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: monomeric orange fluorescent protein with a large Stokes shift (Shcherbakova et al., 2012)
mammalian codon-optimized
ORF:  1 .. 711  =  711 bp
ORF:  236 amino acids  =  26.7 kDa
ORF:  1 .. 711  =  711 bp
ORF:  237 amino acids  =  23.5 kDa  (no start codon)
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Download LSSmOrange.dna file

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