Medium-FT

Blue-to-red fluorescent timer derivative of mCherry with a medium chromophore maturation rate.

Sequence Author: Verkhusha Lab

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No matches
600 400 200 End (711) TatI - BsrGI (701) NmeAIII (689) XcmI (687) BsrFI - SgrAI (682) BsaBI (613) MslI (607) HincII (600) EcoP15I (549) DrdI (541) BbvCI - Bpu10I (539) AlwNI (507) BsrBI (459) StuI (448) PspFI (444) PflMI BseYI (440) BbsI (436) NcoI (434) MmeI (431) PstI - SbfI (356) SfcI (352) BsaAI (231) EcoO109I * (224) AhdI (195) BssSI - BssSαI (88) BanII (76) BsiHKAI (65) ApaLI (61) ApoI - EcoRI (43) MscI (31) Start (0) Medium-FT Medium-FT 711 bp
End  (711)
0 sites
TatI  (701)
1 site
W G T A C W W C A T G W
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NmeAIII  (689)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XcmI  (687)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (682)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (682)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BsaBI  (613)
1 site
G A T N N N N A T C C T A N N N N T A G
MslI  (607)
1 site
C A Y N N N N R T G G T R N N N N Y A C
HincII  (600)
1 site
G T Y R A C C A R Y T G
EcoP15I  (549)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
DrdI  (541)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BbvCI  (539)
1 site
C C T C A G C G G A G T C G
Bpu10I  (539)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AlwNI  (507)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BsrBI  (459)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
StuI  (448)
1 site
A G G C C T T C C G G A
PspFI  (444)
1 site
C C C A G C G G G T C G
PflMI  (440)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BseYI  (440)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (436)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NcoI  (434)
1 site
C C A T G G G G T A C C
MmeI  (431)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (356)
1 site
C T G C A G G A C G T C
SbfI  (356)
1 site
C C T G C A G G G G A C G T C C
SfcI  (352)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaAI  (231)
1 site
Y A C G T R R T G C A Y
EcoO109I  (224)
1 site
R G G N C C Y Y C C N G G R
* Blocked by Dcm methylation.
Sticky ends from different EcoO109I sites may not be compatible.
AhdI  (195)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BssSI  (88)
1 site
C A C G A G G T G C T C
BssSαI  (88)
1 site
C A C G A G G T G C T C
BanII  (76)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (65)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (61)
1 site
G T G C A C C A C G T G
ApoI  (43)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (43)
1 site
G A A T T C C T T A A G
MscI  (31)
1 site
T G G C C A A C C G G T
Start  (0)
0 sites
Medium-FT
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: blue-to-red fluorescent timer derivative of mCherry (Subach et al., 2009)
mammalian codon-optimized
Medium-FT
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: blue-to-red fluorescent timer derivative of mCherry (Subach et al., 2009)
mammalian codon-optimized
ORF:  1 .. 711  =  711 bp
ORF:  236 amino acids  =  26.7 kDa
ORF:  1 .. 711  =  711 bp
ORF:  237 amino acids  =  23.7 kDa  (no start codon)
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