MiCy

Dimeric Midoriishi-Cyan fluorescent protein.

Sequence Author: MBL International

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No matches
600 400 200 End (699) HpaI (678) BbvCI - Bpu10I (665) TaqII (628) StyI (618) AvaI - BsoBI (579) BsrDI (578) BsrFI (533) BstEII (520) BstBI (449) PspFI (438) BseYI (434) MfeI (428) BpmI Eco57MI (354) Esp3I - BsmBI (349) PvuII (332) BseRI (329) NdeI (285) PstI (240) AhdI (189) Bsu36I (155) BclI * (143) EcoNI (116) PvuI - BsiEI (89) ApoI - EcoRI (79) BtsI - BtsαI (69) BtgZI (9) Start (0) MiCy MiCy 699 bp
End  (699)
0 sites
HpaI  (678)
1 site
G T T A A C C A A T T G
BbvCI  (665)
1 site
C C T C A G C G G A G T C G
Bpu10I  (665)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
TaqII  (628)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
StyI  (618)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
AvaI  (579)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (579)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BsrDI  (578)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BsrFI  (533)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BstEII  (520)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstBI  (449)
1 site
T T C G A A A A G C T T
PspFI  (438)
1 site
C C C A G C G G G T C G
BseYI  (434)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
MfeI  (428)
1 site
C A A T T G G T T A A C
BpmI  (354)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (354)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Esp3I  (349)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BsmBI  (349)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
PvuII  (332)
1 site
C A G C T G G T C G A C
BseRI  (329)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
NdeI  (285)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PstI  (240)
1 site
C T G C A G G A C G T C
AhdI  (189)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Bsu36I  (155)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BclI  (143)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
EcoNI  (116)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PvuI  (89)
1 site
C G A T C G G C T A G C
BsiEI  (89)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
ApoI  (79)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (79)
1 site
G A A T T C C T T A A G
BtsI  (69)
1 site
G C A G T G N N C G T C A C
BtsαI  (69)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BtgZI  (9)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
Start  (0)
0 sites
MiCy
1 .. 699  =  699 bp
232 amino acids  =  26.2 kDa
Product: dimeric Midoriishi-Cyan fluorescent protein
MiCy
1 .. 699  =  699 bp
232 amino acids  =  26.2 kDa
Product: dimeric Midoriishi-Cyan fluorescent protein
ORF:  1 .. 699  =  699 bp
ORF:  232 amino acids  =  26.2 kDa
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