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Plasmid Files

Rhacostoma GFP

Dimeric green fluorescent protein from Rhacostoma atlantica.

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Rhacostoma GFP Sequence and MapRhacostoma GFP.dna
Map and Sequence File   
Sequence Author:  Ursa BioScience
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 600 400 200 Rhacostoma GFP End (717) PsiI (701) Bsu36I (635) BsaWI - BspEI (576) BaeGI - Bme1580I * (509) ApaLI (505) TsoI (429) ApoI - EcoRI (427) MfeI (373) BsrGI - TatI (359) BssS α I (326) BsgI (301) AcuI - Eco57MI (290) BsaAI (279) NspI (218) AflIII - PciI (214) MscI (180) EaeI (178) BtgI - NcoI - StyI (175) BsaBI (136) SspI (86) BspQI - EarI - SapI (34) Start (0) Rhacostoma GFP 717 bp
End  (717)
0 sites
PsiI  (701)
1 site
T T A T A A A A T A T T
Bsu36I  (635)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsaWI  (576)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BspEI  (576)
1 site
T C C G G A A G G C C T
BaeGI  (509)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (509)
1 site
G K G C M C C M C G K G
* Blocked by EcoKI methylation.
Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (505)
1 site
G T G C A C C A C G T G
TsoI  (429)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
ApoI  (427)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (427)
1 site
G A A T T C C T T A A G
MfeI  (373)
1 site
C A A T T G G T T A A C
BsrGI  (359)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (359)
1 site
W G T A C W W C A T G W
BssSαI  (326)
1 site
C A C G A G G T G C T C
BsgI  (301)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AcuI  (290)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (290)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (279)
1 site
Y A C G T R R T G C A Y
NspI  (218)
1 site
R C A T G Y Y G T A C R
AflIII  (214)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (214)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
MscI  (180)
1 site
T G G C C A A C C G G T
EaeI  (178)
1 site
Y G G C C R R C C G G Y
BtgI  (175)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (175)
1 site
C C A T G G G G T A C C
StyI  (175)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsaBI  (136)
1 site
G A T N N N N A T C C T A N N N N T A G
SspI  (86)
1 site
A A T A T T T T A T A A
BspQI  (34)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (34)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (34)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
Start  (0)
0 sites
Rhacostoma GFP
1 .. 717  =  717 bp
238 amino acids  =  26.5 kDa
Product: dimeric green fluorescent protein from
Rhacostoma atlantica
Rhacostoma GFP
1 .. 717  =  717 bp
238 amino acids  =  26.5 kDa
Product: dimeric green fluorescent protein from
Rhacostoma atlantica
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