SuperNova

Monomeric variant of the photosensitizing red fluorescent protein KillerRed.
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No matches
600 400 200 End (720) HaeII (698) PmlI - BsaAI (645) MflI * - BstYI (575) NspI (453) PciI (449) PspFI (440) BseYI (436) PvuII - MspA1I (420) BfuAI PaqCI BspMI (358) BanI (310) BsiEI (285) Bpu10I (274) EcoO109I (270) AvaI - BsoBI (265) BanII (212) BsrFI (154) BsiHKAI (140) ApaLI (136) EcoP15I (135) Start (0) SuperNova SuperNova 720 bp
End  (720)
0 sites
HaeII  (698)
1 site
R G C G C Y Y C G C G R
PmlI  (645)
1 site
C A C G T G G T G C A C
BsaAI  (645)
1 site
Y A C G T R R T G C A Y
MflI  (575)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (575)
1 site
R G A T C Y Y C T A G R
NspI  (453)
1 site
R C A T G Y Y G T A C R
PciI  (449)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (440)
1 site
C C C A G C G G G T C G
BseYI  (436)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PvuII  (420)
1 site
C A G C T G G T C G A C
MspA1I  (420)
1 site
C M G C K G G K C G M C
BfuAI  (358)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
PaqCI  (358)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BspMI  (358)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BanI  (310)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BsiEI  (285)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
Bpu10I  (274)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (270)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AvaI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BanII  (212)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsrFI  (154)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsiHKAI  (140)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (136)
1 site
G T G C A C C A C G T G
EcoP15I  (135)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
Start  (0)
0 sites
SuperNova
1 .. 720  =  720 bp
239 amino acids  =  26.5 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: monomeric variant of the photosensitizing red fluorescent protein KillerRed (Takemoto et al., 2013)
SuperNova
1 .. 720  =  720 bp
239 amino acids  =  26.5 kDa
3 segments
   Segment 2:  
   4 .. 9  =  6 bp
   2 amino acids  =  162.1 Da
Product: monomeric variant of the photosensitizing red fluorescent protein KillerRed (Takemoto et al., 2013)
SuperNova
1 .. 720  =  720 bp
239 amino acids  =  26.5 kDa
3 segments
   Segment 3:  
   10 .. 720  =  711 bp
   236 amino acids  =  26.2 kDa
Product: monomeric variant of the photosensitizing red fluorescent protein KillerRed (Takemoto et al., 2013)
SuperNova
1 .. 720  =  720 bp
239 amino acids  =  26.5 kDa
3 segments
Product: monomeric variant of the photosensitizing red fluorescent protein KillerRed (Takemoto et al., 2013)
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.5 kDa
ORF:  3 .. 629  =  627 bp
ORF:  208 amino acids  =  25.2 kDa  (no start codon)
ORF:  7 .. 720  =  714 bp
ORF:  237 amino acids  =  22.7 kDa  (no start codon)
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Download SuperNova.dna file

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