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Plasmid Files

TagGFP2

Monomeric green fluorescent protein. Also known as mTagGFP.

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TagGFP2 Sequence and MapTagGFP2.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 TagGFP2 End (717) TaqII (703) AlwNI (677) EcoP15I (650) Bpu10I (601) BsaBI * (586) PasI (561) PvuII (531) AflIII (446) EciI (445) SacII (327) XmnI (244) BstAPI (206) BstEII (180) BsaHI (107) ApaLI (64) BmgBI (63) BseRI (28) NaeI (27) NgoMIV (25) BsrBI (5) Start (0) TagGFP2 717 bp
End  (717)
0 sites
TaqII  (703)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
AlwNI  (677)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
EcoP15I  (650)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
Bpu10I  (601)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsaBI  (586)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PasI  (561)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PvuII  (531)
1 site
C A G C T G G T C G A C
AflIII  (446)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
EciI  (445)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
SacII  (327)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
XmnI  (244)
1 site
G A A N N N N T T C C T T N N N N A A G
BstAPI  (206)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BstEII  (180)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsaHI  (107)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
ApaLI  (64)
1 site
G T G C A C C A C G T G
BmgBI  (63)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BseRI  (28)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
NaeI  (27)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (25)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BsrBI  (5)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
Start  (0)
0 sites
TagGFP2
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also
known as mTagGFP
mammalian codon-optimized
TagGFP2
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also
known as mTagGFP
mammalian codon-optimized
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