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Plasmid Files

TurboRFP

Red fluorescent protein from Entacmaea quadricolor.

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TurboRFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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600 400 200 End (696) BmrI (691) ScaI (661) MscI (655) EaeI (653) PshAI (628) BsaI (616) DraIII (582) SmlI (559) BpuEI (544) BstYI (535) BfuAI - BspMI (515) Bts α I (510) AcuI - Eco57MI (505) BsgI (493) BglI - SfiI (464) MspA1I (448) PspFI (420) BseYI (416) MmeI (407) BbsI (296) Bsu36I (254) PasI (219) TsoI (163) BsiHKAI (79) ApaLI (75) BsrGI (38) NspI (27) BclI * (11) Start (0) TurboRFP 696 bp
End  (696)
0 sites
BmrI  (691)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
ScaI  (661)
1 site
A G T A C T T C A T G A
MscI  (655)
1 site
T G G C C A A C C G G T
EaeI  (653)
1 site
Y G G C C R R C C G G Y
PshAI  (628)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaI  (616)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
DraIII  (582)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SmlI  (559)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (544)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstYI  (535)
1 site
R G A T C Y Y C T A G R
BfuAI  (515)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (515)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BtsαI  (510)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (505)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsgI  (493)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (464)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (464)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
MspA1I  (448)
1 site
C M G C K G G K C G M C
PspFI  (420)
1 site
C C C A G C G G G T C G
BseYI  (416)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
MmeI  (407)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbsI  (296)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PasI  (219)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
TsoI  (163)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsiHKAI  (79)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (75)
1 site
G T G C A C C A C G T G
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NspI  (27)
1 site
R C A T G Y Y G T A C R
BclI  (11)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
TurboRFP
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
TurboRFP
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
ORF:  1 .. 696  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  3 .. 242  =  240 bp
ORF:  79 amino acids  =  9.1 kDa  (no start codon)
ORF:  1 .. 696  =  696 bp
ORF:  232 amino acids  =  23.8 kDa  (no start codon)
ORF:  102 .. 347  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
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