ZsGreen

Zoanthus green fluorescent protein, also known as zFP506.

Sequence Author: Clontech (TaKaRa)

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No matches
600 400 200 End (696) MflI * - BstYI (679) BsaWI - BspEI * (676) BtgZI (644) BglI (588) BanI (533) AcuI - Eco57MI (532) EcoNI (511) Bpu10I (467) BmrI (441) BspHI (363) NdeI (316) TsoI (257) BssSI BssSαI (255) SmlI (227) BpuEI (212) PvuII - MspA1I (187) AhdI (180) AseI (131) EcoRV (106) BclI * (83) Start (0) ZsGreen ZsGreen 696 bp
End  (696)
0 sites
MflI  (679)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (679)
1 site
R G A T C Y Y C T A G R
BsaWI  (676)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
BspEI  (676)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
BtgZI  (644)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BglI  (588)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BanI  (533)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
AcuI  (532)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (532)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoNI  (511)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Bpu10I  (467)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BmrI  (441)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BspHI  (363)
1 site
T C A T G A A G T A C T
NdeI  (316)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
TsoI  (257)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BssSI  (255)
1 site
C A C G A G G T G C T C
BssSαI  (255)
1 site
C A C G A G G T G C T C
SmlI  (227)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (212)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuII  (187)
1 site
C A G C T G G T C G A C
MspA1I  (187)
1 site
C M G C K G G K C G M C
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AseI  (131)
1 site
A T T A A T T A A T T A
EcoRV  (106)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BclI  (83)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
ZsGreen
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein (Matz et al., 1999)
ZsGreen
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein (Matz et al., 1999)
ORF:  1 .. 696  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
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Download ZsGreen.dna file

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