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Plasmid Files

ZsGreen

Zoanthus green fluorescent protein, also known as zFP506.

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ZsGreen Sequence and MapZsGreen.dna
Map and Sequence File   
Sequence Author:  Clontech
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 600 400 200 ZsGreen End (696) BstYI (679) BsaWI - BspEI * (676) BtgZI (644) BglI (588) BanI (533) AcuI - Eco57MI (532) EcoNI (511) Bpu10I (467) BmrI (441) BspHI (363) NdeI (316) TsoI (257) BssS α I (255) SmlI (227) BpuEI (212) MspA1I - PvuII (187) AhdI (180) AseI (131) EcoRV (106) BclI * (83) Start (0) ZsGreen 696 bp
End  (696)
0 sites
BstYI  (679)
1 site
R G A T C Y Y C T A G R
BsaWI  (676)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BspEI  (676)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
BtgZI  (644)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BglI  (588)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BanI  (533)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
AcuI  (532)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (532)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoNI  (511)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Bpu10I  (467)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BmrI  (441)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
BspHI  (363)
1 site
T C A T G A A G T A C T
NdeI  (316)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
TsoI  (257)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BssSαI  (255)
1 site
C A C G A G G T G C T C
SmlI  (227)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (212)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MspA1I  (187)
1 site
C M G C K G G K C G M C
PvuII  (187)
1 site
C A G C T G G T C G A C
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AseI  (131)
1 site
A T T A A T T A A T T A
EcoRV  (106)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BclI  (83)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
ZsGreen
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein (Matz
et al., 1999)
ZsGreen
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein (Matz
et al., 1999)
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