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Plasmid Files

ZsYellow1

Zoanthus yellow fluorescent protein.

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ZsYellow1 Sequence and MapZsYellow1.dna
Map and Sequence File   
Sequence Author:  Clontech
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 600 400 200 ZsYellow1 End (696) BstXI (687) TaqII (673) BsiHKAI (665) BtgZI (654) BstAPI (609) BmeT110I (584) AvaI - BsoBI (583) BaeGI - Bme1580I (572) BsrGI - TatI (551) KpnI (531) AgeI (530) Acc65I (527) EagI (522) BfuAI - BspMI (512) NspI (501) BmgBI (492) BmrI (441) PflMI (431) PluTI (308) SfoI (306) NarI (305) KasI (304) BstYI (209) SgrAI (88) BclI * (83) BseRI (43) Start (0) ZsYellow1 696 bp
End  (696)
0 sites
BstXI  (687)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
TaqII  (673)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BsiHKAI  (665)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BtgZI  (654)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BstAPI  (609)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BmeT110I  (584)
1 site
C Y C G R G G R G C Y C
AvaI  (583)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (583)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BaeGI  (572)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (572)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsrGI  (551)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (551)
1 site
W G T A C W W C A T G W
KpnI  (531)
1 site
G G T A C C C C A T G G
AgeI  (530)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
Acc65I  (527)
1 site
G G T A C C C C A T G G
EagI  (522)
1 site
C G G C C G G C C G G C
BfuAI  (512)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (512)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
NspI  (501)
1 site
R C A T G Y Y G T A C R
BmgBI  (492)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BmrI  (441)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
PflMI  (431)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
PluTI  (308)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (306)
1 site
G G C G C C C C G C G G
NarI  (305)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (304)
1 site
G G C G C C C C G C G G
BstYI  (209)
1 site
R G A T C Y Y C T A G R
SgrAI  (88)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BclI  (83)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BseRI  (43)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
ZsYellow1
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus yellow fluorescent protein
mammalian codon-optimized
ZsYellow1
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus yellow fluorescent protein
mammalian codon-optimized
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