Resources
Plasmid Files

d1EGFP

EGFP destabilized by a mutated version of residues 422-461 of mouse ornithine decarboxylase, giving an in vivo half-life of ~1 hour.

To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

d1EGFP Sequence and Mapd1EGFP.dna
Map and Sequence File   
Sequence Author:  Clontech
Download Free Trial Get SnapGene Viewer

 800 600 400 200 EGFP destabilizati... d1EGFP End (846) BstAPI (820) PstI (816) SfcI (812) BsrBI (794) NaeI (740) TsoI (739) MreI - NgoMIV - SgrAI (738) BtgI - NcoI - StyI (726) BlpI (721) HindIII (718) BsrGI (710) TaqII (625) BsiHKAI (610) BmrI (606) BstYI (500) PfoI * (281) Bts α I (214) BssS α I (181) BtgZI (122) BanI (36) BseRI (31) Start (0) d1EGFP 846 bp
End  (846)
0 sites
BstAPI  (820)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PstI  (816)
1 site
C T G C A G G A C G T C
SfcI  (812)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BsrBI  (794)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
NaeI  (740)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
TsoI  (739)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
MreI  (738)
1 site
C G C C G G C G G C G G C C G C
NgoMIV  (738)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
SgrAI  (738)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BtgI  (726)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (726)
1 site
C C A T G G G G T A C C
StyI  (726)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BlpI  (721)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
HindIII  (718)
1 site
A A G C T T T T C G A A
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TaqII  (625)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BsiHKAI  (610)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BmrI  (606)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BssSαI  (181)
1 site
C A C G A G G T G C T C
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
d1EGFP
1 .. 846  =  846 bp
281 amino acids  =  31.2 kDa
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: EGFP destabilized by fusion to a mutated
version of residues 422-461 of mouse ornithine
decarboxylase, giving an in vivo half-life of ~1 hour
mammalian codon-optimized
d1EGFP
1 .. 846  =  846 bp
281 amino acids  =  31.2 kDa
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.2 Da
Product: EGFP destabilized by fusion to a mutated
version of residues 422-461 of mouse ornithine
decarboxylase, giving an in vivo half-life of ~1 hour
mammalian codon-optimized
d1EGFP
1 .. 846  =  846 bp
281 amino acids  =  31.2 kDa
   Segment 3:  EGFP  
   7 .. 717  =  711 bp
   237 amino acids  =  26.7 kDa
Product: EGFP destabilized by fusion to a mutated
version of residues 422-461 of mouse ornithine
decarboxylase, giving an in vivo half-life of ~1 hour
mammalian codon-optimized
d1EGFP
1 .. 846  =  846 bp
281 amino acids  =  31.2 kDa
   Segment 4:  
   718 .. 723  =  6 bp
   2 amino acids  =  259.3 Da
Product: EGFP destabilized by fusion to a mutated
version of residues 422-461 of mouse ornithine
decarboxylase, giving an in vivo half-life of ~1 hour
mammalian codon-optimized
d1EGFP
1 .. 846  =  846 bp
281 amino acids  =  31.2 kDa
   Segment 5:  destabilization domain  
   724 .. 846  =  123 bp
   40 amino acids  =  4.1 kDa
Product: EGFP destabilized by fusion to a mutated
version of residues 422-461 of mouse ornithine
decarboxylase, giving an in vivo half-life of ~1 hour
mammalian codon-optimized
d1EGFP
1 .. 846  =  846 bp
281 amino acids  =  31.2 kDa
5 segments
Product: EGFP destabilized by fusion to a mutated
version of residues 422-461 of mouse ornithine
decarboxylase, giving an in vivo half-life of ~1 hour
mammalian codon-optimized
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter