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Plasmid Files

daGFP

Green fluorescent protein with peak excitation at 510 nm and peak emission at 521 nm.

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daGFP Sequence and MapdaGFP.dna
Map and Sequence File   
Sequence Author:  Oxford Genetics
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 600 400 200 daGFP End (711) NdeI (659) EcoP15I (608) PfoI (461) MslI (432) EciI (428) BstAPI (411) BmrI (389) BsiWI (341) EaeI (260) NruI (223) PasI (176) BtgI (155) BsiEI (125) BsaWI (124) ApoI (85) MmeI (41) XmnI (28) Start (0) daGFP 711 bp
End  (711)
0 sites
NdeI  (659)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
EcoP15I  (608)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
PfoI  (461)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
MslI  (432)
1 site
C A Y N N N N R T G G T R N N N N Y A C
EciI  (428)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BstAPI  (411)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BmrI  (389)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
BsiWI  (341)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
EaeI  (260)
1 site
Y G G C C R R C C G G Y
NruI  (223)
1 site
T C G C G A A G C G C T
PasI  (176)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BtgI  (155)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsiEI  (125)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
BsaWI  (124)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
ApoI  (85)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
MmeI  (41)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XmnI  (28)
1 site
G A A N N N N T T C C T T N N N N A A G
Start  (0)
0 sites
daGFP
1 .. 711  =  711 bp
236 amino acids  =  26.6 kDa
Product: green fluorescent protein with peak
excitation at 510 nm and peak emission at 521 nm
daGFP
1 .. 711  =  711 bp
236 amino acids  =  26.6 kDa
Product: green fluorescent protein with peak
excitation at 510 nm and peak emission at 521 nm
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