Resources
Plasmid Files

hKikGR1

Humanized photoconvertible Kikume green-to-red fluorescent protein.

To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

hKikGR1 Sequence and MaphKikGR1.dna
Map and Sequence File   
Sequence Author:  MBL International
Download Free Trial Get SnapGene Viewer

 600 400 200 End (687) StyI (650) BsiHKAI (647) DraIII (582) BsgI (580) BstXI (555) BpmI (484) BmgBI (480) EcoP15I (465) BsaAI (453) BanII (434) BbsI (421) AlwNI - BstAPI (347) MslI (325) BsrFI (79) HaeII - PluTI (53) SfoI (51) BsaHI - NarI (50) BanI - KasI (49) BbvCI - Bpu10I (37) Start (0) hKikGR1 687 bp
End  (687)
0 sites
StyI  (650)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsiHKAI  (647)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
DraIII  (582)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsgI  (580)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstXI  (555)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BpmI  (484)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmgBI  (480)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
EcoP15I  (465)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BsaAI  (453)
1 site
Y A C G T R R T G C A Y
BanII  (434)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AlwNI  (347)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstAPI  (347)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BsrFI  (79)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
HaeII  (53)
1 site
R G C G C Y Y C G C G R
PluTI  (53)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (51)
1 site
G G C G C C C C G C G G
BsaHI  (50)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (50)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
BanI  (49)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
KasI  (49)
1 site
G G C G C C C C G C G G
BbvCI  (37)
1 site
C C T C A G C G G A G T C G
Bpu10I  (37)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Start  (0)
0 sites
hKikGR1
1 .. 687  =  687 bp
228 amino acids  =  25.9 kDa
Product: humanized photoconvertible Kikume
green-to-red fluorescent protein
mammalian codon-optimized
hKikGR1
1 .. 687  =  687 bp
228 amino acids  =  25.9 kDa
Product: humanized photoconvertible Kikume
green-to-red fluorescent protein
mammalian codon-optimized
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter